BMS.serial_dilution_volumes() Function¶

This function can be helpful when writing protocols with a serial dilution step. By defining the serial dilution steps and the final volume for each dilution, the BMS.serial_dilution_volumes function can calculate the volume of sample and solution required at each step.

Usage¶

First, the BMS generic tools module is imported as BMS

In [1]:

import BiomationScripter as BMS

import BiomationScripter as BMS

Next, the dilution factors for the serial dilution should be specified. The example below shows a 1 in 2 serial dilution with 5 steps, plus an undiluted step

In [2]:

Dilution_Factors = [1, 2, 4, 8, 16, 32]

Dilution_Factors = [1, 2, 4, 8, 16, 32]

Next, the final volume for each dilution should be specified in microlitres

In [3]:

Final_Volume = 100

Final_Volume = 100

Finally, the BMS.serial_dilution_volumes function can be used to calculate and return the volumes of sample and solution required

In [4]:

Sample_Volumes, Solution_Volumes = BMS.serial_dilution_volumes(
    dilution_factors = Dilution_Factors,
    total_volume= Final_Volume
)
print(Sample_Volumes)
print(Solution_Volumes)

Sample_Volumes, Solution_Volumes = BMS.serial_dilution_volumes( dilution_factors = Dilution_Factors, total_volume= Final_Volume ) print(Sample_Volumes) print(Solution_Volumes)

[100.0, 50.0, 50.0, 50.0, 50.0, 50.0]
[0.0, 50.0, 50.0, 50.0, 50.0, 50.0]

The order of the volumes in each list above are determined by the order of the dilution factors used as the input

It is also possible to define irregular serial dilutions

In [5]:

Dilution_Factors = [1, 2, 10, 50, 75, 100, 200]
Final_Volume = 100

Dilution_Factors = [1, 2, 10, 50, 75, 100, 200] Final_Volume = 100

In [6]:

Sample_Volumes, Solution_Volumes = BMS.serial_dilution_volumes(
    dilution_factors = Dilution_Factors,
    total_volume= Final_Volume
)
print(Sample_Volumes)
print(Solution_Volumes)

Sample_Volumes, Solution_Volumes = BMS.serial_dilution_volumes( dilution_factors = Dilution_Factors, total_volume= Final_Volume ) print(Sample_Volumes) print(Solution_Volumes)

[100.0, 50.0, 20.0, 20.0, 66.66666666666666, 75.0, 50.0]
[0.0, 50.0, 80.0, 80.0, 33.33333333333334, 25.0, 50.0]