Writing an OT2 Protocol using OTProto¶

The generic BMS and OTProto tools can be used to help write automation protocols for the Opentrons. If you will be running the same protocol a lot but with variations, you may want to look at creating an OTProto template (if one doesn't already exist). However to begin with, or for protocols which will not need to be modified with each run, it can be more useful and faster to write the protocol as a script, rather than as a template.

The tutorial here will talk through the creation of an automation protocol for setting up a protocol to mix together different types of food colouring in a 96 well plate.

Defining the Protocol¶

The first step is to determine what exactly our protocol will be doing.

Protocol behaviour¶

This protocol will combinatorially create two-colour mixtures of food colouring from a stock of four different food colouring with a final volume of 100 uL. Each two-colour mixture will be made in triplicate.

Labware and materials¶

The protocol will use four types of food colouring:

Green
Blue
Red
Purple

The food colouring will be supplied in 1 mL tubes. The tubes will be stored in a 24 well aluminium tube rack.

The food colouring mixtures will be prepared into a Greiner 96 well flat-bottom plate.

Standard Opentrons 300 uL tips will be used.

Setting Up¶

The first step is to import the BMS generic tools and the OTProto module:

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import BiomationScripter as BMS
import BiomationScripter.OTProto as OTP
import BiomationScripter as BMS
import BiomationScripter.OTProto as OTP

We'll also import the math module, as we'll use it later on

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import math
import math

This next step is not needed for simulating the protocol on a computer, but is required for running the protocol on the Opentrons. This step is used to point the Opentrons to the location of the BMS library. To see how BMS is loaded onto the Opentrons, see here.

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import sys
sys.path.insert(0, "/var/lib/jupyter/notebooks/Packages/BiomationScripterLib")
import sys
sys.path.insert(0, "/var/lib/jupyter/notebooks/Packages/BiomationScripterLib")

Finally, the metadata for the protocol needs to be defined. This is done through the use of a Python dictionary, as shown below:

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metadata = {
    'protocolName': 'Colour Mixing Tutorial',
    'description': 'A protocol to create combinatorial mixtures of food colouring.',
    'author': 'Bradley Brown',
    'author-email': 'b.bradley2@newcastle.ac.uk',
    'user': 'Your Name',
    'user-email': 'name.your@email.co.uk',
    'source': 'BiomationScripter Library',
    'bms-version': '1.0.0',
    'apiLevel': '2.11',
    'robotName': 'RoBoT2' # This is the name of the OT2 you plan to run the protocol on
}
metadata = {
    'protocolName': 'Colour Mixing Tutorial',
    'description': 'A protocol to create combinatorial mixtures of food colouring.',
    'author': 'Bradley Brown',
    'author-email': 'b.bradley2@newcastle.ac.uk',
    'user': 'Your Name',
    'user-email': 'name.your@email.co.uk',
    'source': 'BiomationScripter Library',
    'bms-version': '1.0.0',
    'apiLevel': '2.11',
    'robotName': 'RoBoT2' # This is the name of the OT2 you plan to run the protocol on
}

Making the `run` Function¶

The rest of the Opentrons protocol must be contained within a single Python function namedrun, which takes an argument with keyword protcol. This argument accepts the ProtocolContext object generated by the Opentrons. You don't need to worry too much about how this is working for now, but you can read more here.

For now, we will define the function and have it do nothing:

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def run(protocol):
    pass
def run(protocol):
    pass

NOTE: To make the walkthrough easier to follow, the code will be shown outside of this function. It will then be packaged back into the function at the end.

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from opentrons import simulate as OT2 # This line simulates the protocol
# Get the correct api version
protocol = OT2.get_protocol_api(metadata["apiLevel"])
# Home the pipetting head
protocol.home()
from opentrons import simulate as OT2 # This line simulates the protocol
# Get the correct api version
protocol = OT2.get_protocol_api(metadata["apiLevel"])
# Home the pipetting head
protocol.home()

C:\Users\bradl\.opentrons\robot_settings.json not found. Loading defaults
C:\Users\bradl\.opentrons\deck_calibration.json not found. Loading defaults

General Information¶

The first thing we'll do is define some of the general information for the protocol. This includes a list of source materials and their aliquot volumes, the pipettes types available, and the types of tips which will be used.

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#######################
# General Information #
#######################

# Define the types of pipettes, and their location
Pipettes = {
    "left": "p20_single_gen2",
    "right": "p300_single_gen2"
}

# For each pipette, define the pipette tips available to them
Pipette_Tips = {
    "left": "opentrons_96_tiprack_20ul",
    "right": "opentrons_96_tiprack_300ul"
}

# List of source material

Food_Colouring = [
    "Green",
    "Blue",
    "Red",
    "Purple"
]

Food_Colouring_Aliquot_Volume = 500 # uL
#######################
# General Information #
#######################

# Define the types of pipettes, and their location
Pipettes = {
    "left": "p20_single_gen2",
    "right": "p300_single_gen2"
}

# For each pipette, define the pipette tips available to them
Pipette_Tips = {
    "left": "opentrons_96_tiprack_20ul",
    "right": "opentrons_96_tiprack_300ul"
}

# List of source material

Food_Colouring = [
    "Green",
    "Blue",
    "Red",
    "Purple"
]

Food_Colouring_Aliquot_Volume = 500 # uL

Load Pipettes¶

Here we'll load the pipettes - this lets the Opentrons API know which pipettes are available, and which position they're in. Note that this code uses the protocol object. This is the object passed in by the run function mentioned above. So for now, running this will cause an error (as we haven't defined protocol). This will become functional once we construct the whole function at the end.

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#################
# Load Pipettes #
#################

for position in Pipettes.keys():
    protocol.load_instrument(
        Pipettes[position],
        position
    )
#################
# Load Pipettes #
#################

for position in Pipettes.keys():
    protocol.load_instrument(
        Pipettes[position],
        position
    )

Definining the Labware¶

Here we will define the labware (i.e the plates, tubes, and racks) required by the protocol, as defined above. It is important to determine whether you are using any 'custom' labware. Custom labware simply refers to any labware which is not included in the Opentrons Labware Library.

In this protocol, not including the pipette tips which will be dealt with later, we are using two types of labware:

Aluminium 24 well tube rack: used to host the source materials (i.e. the food colouring)
Greiner 96 well plate: used to prepare the serial dilutions

The tube rack is included in the labware library (found here), and so is not custom labware.

the Greiner 96 well plate does not exist in the labware library, and so is referred to as custom, and requires a definition file. Definition files are json files which let the Opentrons know the dimensions of the labware (in this case, a 96 well plate).

For custom labware, the Opentrons Labware Creator can be used to create definition files. Once you've created the custom labware file, follow the instructions on the creator to calibrate the labware on your robot.

For this tutorial, we have already created the definition file, which can be found here.

To simulate the protocol, we need to supply the location of any custom labware files. This is shown below:

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##################
# Set up labware #
##################
Custom_Labware_Directory = "../../../../data/custom_labware"
##################
# Set up labware #
##################
Custom_Labware_Directory = "../../../../data/custom_labware"

Then we will define the labware types using their API name. The API name is an identifier used by the Opentrons to get the desired labware. For non-custom labware, such as the aluminium tube rack, this can be found in the Opentrons Labware Library. For custom labware, like the 96 well plate, this is the name of the definition file, without the '.json' extension. So in this case, the API names would be:

opentrons_24_aluminumblock_nest_1.5ml_snapcap
greiner655087_96_wellplate_340ul

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##################
# Set up labware #
##################
Custom_Labware_Directory = "../../../../data/custom_labware"

Tube_Rack_Type = "opentrons_24_aluminumblock_nest_1.5ml_snapcap"
Destination_Type = "greiner655087_96_wellplate_340ul"
##################
# Set up labware #
##################
Custom_Labware_Directory = "../../../../data/custom_labware"

Tube_Rack_Type = "opentrons_24_aluminumblock_nest_1.5ml_snapcap"
Destination_Type = "greiner655087_96_wellplate_340ul"

Now we'll make use of the BMS.Labware_Layout class to help keep track of what's in our source and destination labware. This can be used later to simplify some of the liquid handling steps.

Let's first create a Labware_Layout object for our source labware.

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##########################
# Set up labware layouts #
##########################

# Create the source object
Source_Labware_Layout = BMS.Labware_Layout(
    Name = "Source Labware",
    Type = Tube_Rack_Type
)
##########################
# Set up labware layouts #
##########################

# Create the source object
Source_Labware_Layout = BMS.Labware_Layout(
    Name = "Source Labware",
    Type = Tube_Rack_Type
)

Next we'll define the format of the labware (i.e. the number of rows and columns). We can use the OTP.get_labware_format function to get the labware format, and then store it in the Labware_Layout object with the .define_format method.

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##########################
# Set up labware layouts #
##########################

# Create the source object
Source_Labware_Layout = BMS.Labware_Layout(
    Name = "Source Labware",
    Type = Tube_Rack_Type
)

# Define the labware's format (i.e. number of rows and columns)
source_rows, source_columns = OTP.get_labware_format(
    labware_api_name = Source_Labware_Layout.type,
)
Source_Labware_Layout.define_format(source_rows, source_columns)
##########################
# Set up labware layouts #
##########################

# Create the source object
Source_Labware_Layout = BMS.Labware_Layout(
    Name = "Source Labware",
    Type = Tube_Rack_Type
)

# Define the labware's format (i.e. number of rows and columns)
source_rows, source_columns = OTP.get_labware_format(
    labware_api_name = Source_Labware_Layout.type,
)
Source_Labware_Layout.define_format(source_rows, source_columns)

Then we do the same for the destination labware. Note that this time we need to supply OTP.get_labware_format with the directory (folder) containing the labware definition file. This is because the destination labware is custom labware.

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# Create the destination object
Destination_Labware_Layout = BMS.Labware_Layout(
    Name = "Destination Labware",
    Type = Destination_Type
)

# Define the labware's format (i.e. number of rows and columns)
destination_rows, destination_columns = OTP.get_labware_format(
    labware_api_name = Destination_Labware_Layout.type,
    custom_labware_dir = Custom_Labware_Directory
)
Destination_Labware_Layout.define_format(destination_rows, destination_columns)
# Create the destination object
Destination_Labware_Layout = BMS.Labware_Layout(
    Name = "Destination Labware",
    Type = Destination_Type
)

# Define the labware's format (i.e. number of rows and columns)
destination_rows, destination_columns = OTP.get_labware_format(
    labware_api_name = Destination_Labware_Layout.type,
    custom_labware_dir = Custom_Labware_Directory
)
Destination_Labware_Layout.define_format(destination_rows, destination_columns)

We can also specify which wells in the labware are available for use. This can be useful in cases where some labware, like plates, are being re-used from previous protocols, and therefore some wells are contaminated.

For this example, we'll assume that all wells for all labware are available. Information on how the .set_available_wells() method works can be found in the documentation.

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Source_Labware_Layout.set_available_wells()
Destination_Labware_Layout.set_available_wells()
Source_Labware_Layout.set_available_wells()
Destination_Labware_Layout.set_available_wells()

Define the Destination State¶

In this section, we'll define the final state of the destination labware. First, let's write some code to get a list of all the different two-colour mixtures which exist.

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################################
# Define the destination state #
################################

# Set up an empty list in which the colour mixtures required will be added
Colour_Mixtures = []

# Iterate through the different ratios
# Iterate through the list of source colours provided to create the list of mixtures.
for colour_1 in Food_Colouring:
    for colour_2 in Food_Colouring:
        # Ignore situations where colour_1 and colour_2 are the same
        if colour_1 == colour_2:
            continue
        else:
            # Add the two colours to the list of mixtures to prepare
            Colour_Mixtures.append([colour_1, colour_2])

# Here, we'll print to OUT all of the mixtures which will be prepared
for c in Colour_Mixtures:
    print(c)
################################
# Define the destination state #
################################

# Set up an empty list in which the colour mixtures required will be added
Colour_Mixtures = []

# Iterate through the different ratios
# Iterate through the list of source colours provided to create the list of mixtures.
for colour_1 in Food_Colouring:
    for colour_2 in Food_Colouring:
        # Ignore situations where colour_1 and colour_2 are the same
        if colour_1 == colour_2:
            continue
        else:
            # Add the two colours to the list of mixtures to prepare
            Colour_Mixtures.append([colour_1, colour_2])

# Here, we'll print to OUT all of the mixtures which will be prepared
for c in Colour_Mixtures:
    print(c)

['Green', 'Blue']
['Green', 'Red']
['Green', 'Purple']
['Blue', 'Green']
['Blue', 'Red']
['Blue', 'Purple']
['Red', 'Green']
['Red', 'Blue']
['Red', 'Purple']
['Purple', 'Green']
['Purple', 'Blue']
['Purple', 'Red']

Now we can use this list of mixtures to define the final state of the destination labware. Note that Bbcause we've defined which wells are available, we can use the get_next_empty_well method, which will simply return the next available well with no content.

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# Define the final destination state

# For 3 replicates
for rep in range(0, 3):
    # For each mixture
    for colour_1, colour_2 in Colour_Mixtures:
        
        # Get the next empty well
        well = Destination_Labware_Layout.get_next_empty_well()

        # Add the first colourant
        Destination_Labware_Layout.add_content(
            Well = well,
            Reagent = colour_1,
            Volume = 50 # uL
        )
        
        # And then the second
        Destination_Labware_Layout.add_content(
            Well = well,
            Reagent = colour_2,
            Volume = 50 # uL
        )
        
        # Can also label the well
        Destination_Labware_Layout.add_well_label(
            Well = well,
            Label = "Mixture: {}-{} Rep {}".format(colour_1, colour_2, rep)
        )
# Define the final destination state

# For 3 replicates
for rep in range(0, 3):
    # For each mixture
    for colour_1, colour_2 in Colour_Mixtures:
        
        # Get the next empty well
        well = Destination_Labware_Layout.get_next_empty_well()

        # Add the first colourant
        Destination_Labware_Layout.add_content(
            Well = well,
            Reagent = colour_1,
            Volume = 50 # uL
        )
        
        # And then the second
        Destination_Labware_Layout.add_content(
            Well = well,
            Reagent = colour_2,
            Volume = 50 # uL
        )
        
        # Can also label the well
        Destination_Labware_Layout.add_well_label(
            Well = well,
            Label = "Mixture: {}-{} Rep {}".format(colour_1, colour_2, rep)
        )

At any point, the .print method can be used to print to OUT information about a labware layout and its content.

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Destination_Labware_Layout.print()
Destination_Labware_Layout.print()

Information for Destination Labware
Plate Type: greiner655087_96_wellplate_340ul
Well	Volume(uL)	Liquid Class	Reagent
A1	50.0		Unknown		Green
A1	50.0		Unknown		Blue
A2	50.0		Unknown		Green
A2	50.0		Unknown		Red
A3	50.0		Unknown		Green
A3	50.0		Unknown		Purple
A4	50.0		Unknown		Blue
A4	50.0		Unknown		Green
A5	50.0		Unknown		Blue
A5	50.0		Unknown		Red
A6	50.0		Unknown		Blue
A6	50.0		Unknown		Purple
A7	50.0		Unknown		Red
A7	50.0		Unknown		Green
A8	50.0		Unknown		Red
A8	50.0		Unknown		Blue
A9	50.0		Unknown		Red
A9	50.0		Unknown		Purple
A10	50.0		Unknown		Purple
A10	50.0		Unknown		Green
A11	50.0		Unknown		Purple
A11	50.0		Unknown		Blue
A12	50.0		Unknown		Purple
A12	50.0		Unknown		Red
B1	50.0		Unknown		Green
B1	50.0		Unknown		Blue
B2	50.0		Unknown		Green
B2	50.0		Unknown		Red
B3	50.0		Unknown		Green
B3	50.0		Unknown		Purple
B4	50.0		Unknown		Blue
B4	50.0		Unknown		Green
B5	50.0		Unknown		Blue
B5	50.0		Unknown		Red
B6	50.0		Unknown		Blue
B6	50.0		Unknown		Purple
B7	50.0		Unknown		Red
B7	50.0		Unknown		Green
B8	50.0		Unknown		Red
B8	50.0		Unknown		Blue
B9	50.0		Unknown		Red
B9	50.0		Unknown		Purple
B10	50.0		Unknown		Purple
B10	50.0		Unknown		Green
B11	50.0		Unknown		Purple
B11	50.0		Unknown		Blue
B12	50.0		Unknown		Purple
B12	50.0		Unknown		Red
C1	50.0		Unknown		Green
C1	50.0		Unknown		Blue
C2	50.0		Unknown		Green
C2	50.0		Unknown		Red
C3	50.0		Unknown		Green
C3	50.0		Unknown		Purple
C4	50.0		Unknown		Blue
C4	50.0		Unknown		Green
C5	50.0		Unknown		Blue
C5	50.0		Unknown		Red
C6	50.0		Unknown		Blue
C6	50.0		Unknown		Purple
C7	50.0		Unknown		Red
C7	50.0		Unknown		Green
C8	50.0		Unknown		Red
C8	50.0		Unknown		Blue
C9	50.0		Unknown		Red
C9	50.0		Unknown		Purple
C10	50.0		Unknown		Purple
C10	50.0		Unknown		Green
C11	50.0		Unknown		Purple
C11	50.0		Unknown		Blue
C12	50.0		Unknown		Purple
C12	50.0		Unknown		Red

Out[17]:

'A1\t50.0\t\tUnknown\t\tGreen\nA1\t50.0\t\tUnknown\t\tBlue\nA2\t50.0\t\tUnknown\t\tGreen\nA2\t50.0\t\tUnknown\t\tRed\nA3\t50.0\t\tUnknown\t\tGreen\nA3\t50.0\t\tUnknown\t\tPurple\nA4\t50.0\t\tUnknown\t\tBlue\nA4\t50.0\t\tUnknown\t\tGreen\nA5\t50.0\t\tUnknown\t\tBlue\nA5\t50.0\t\tUnknown\t\tRed\nA6\t50.0\t\tUnknown\t\tBlue\nA6\t50.0\t\tUnknown\t\tPurple\nA7\t50.0\t\tUnknown\t\tRed\nA7\t50.0\t\tUnknown\t\tGreen\nA8\t50.0\t\tUnknown\t\tRed\nA8\t50.0\t\tUnknown\t\tBlue\nA9\t50.0\t\tUnknown\t\tRed\nA9\t50.0\t\tUnknown\t\tPurple\nA10\t50.0\t\tUnknown\t\tPurple\nA10\t50.0\t\tUnknown\t\tGreen\nA11\t50.0\t\tUnknown\t\tPurple\nA11\t50.0\t\tUnknown\t\tBlue\nA12\t50.0\t\tUnknown\t\tPurple\nA12\t50.0\t\tUnknown\t\tRed\nB1\t50.0\t\tUnknown\t\tGreen\nB1\t50.0\t\tUnknown\t\tBlue\nB2\t50.0\t\tUnknown\t\tGreen\nB2\t50.0\t\tUnknown\t\tRed\nB3\t50.0\t\tUnknown\t\tGreen\nB3\t50.0\t\tUnknown\t\tPurple\nB4\t50.0\t\tUnknown\t\tBlue\nB4\t50.0\t\tUnknown\t\tGreen\nB5\t50.0\t\tUnknown\t\tBlue\nB5\t50.0\t\tUnknown\t\tRed\nB6\t50.0\t\tUnknown\t\tBlue\nB6\t50.0\t\tUnknown\t\tPurple\nB7\t50.0\t\tUnknown\t\tRed\nB7\t50.0\t\tUnknown\t\tGreen\nB8\t50.0\t\tUnknown\t\tRed\nB8\t50.0\t\tUnknown\t\tBlue\nB9\t50.0\t\tUnknown\t\tRed\nB9\t50.0\t\tUnknown\t\tPurple\nB10\t50.0\t\tUnknown\t\tPurple\nB10\t50.0\t\tUnknown\t\tGreen\nB11\t50.0\t\tUnknown\t\tPurple\nB11\t50.0\t\tUnknown\t\tBlue\nB12\t50.0\t\tUnknown\t\tPurple\nB12\t50.0\t\tUnknown\t\tRed\nC1\t50.0\t\tUnknown\t\tGreen\nC1\t50.0\t\tUnknown\t\tBlue\nC2\t50.0\t\tUnknown\t\tGreen\nC2\t50.0\t\tUnknown\t\tRed\nC3\t50.0\t\tUnknown\t\tGreen\nC3\t50.0\t\tUnknown\t\tPurple\nC4\t50.0\t\tUnknown\t\tBlue\nC4\t50.0\t\tUnknown\t\tGreen\nC5\t50.0\t\tUnknown\t\tBlue\nC5\t50.0\t\tUnknown\t\tRed\nC6\t50.0\t\tUnknown\t\tBlue\nC6\t50.0\t\tUnknown\t\tPurple\nC7\t50.0\t\tUnknown\t\tRed\nC7\t50.0\t\tUnknown\t\tGreen\nC8\t50.0\t\tUnknown\t\tRed\nC8\t50.0\t\tUnknown\t\tBlue\nC9\t50.0\t\tUnknown\t\tRed\nC9\t50.0\t\tUnknown\t\tPurple\nC10\t50.0\t\tUnknown\t\tPurple\nC10\t50.0\t\tUnknown\t\tGreen\nC11\t50.0\t\tUnknown\t\tPurple\nC11\t50.0\t\tUnknown\t\tBlue\nC12\t50.0\t\tUnknown\t\tPurple\nC12\t50.0\t\tUnknown\t\tRed\n'

Set up the Source Labware¶

Here, we'll determine how much of each source material (in this case the food colourings) is required to run the protocol, and then add the required sources to the source labware layout.

For each food colouring type, we'll search the Destination labware layout and find out the total volume required for that colourant. Then, we'll divide that by the aliquot volume to find the number of aliquots needed, and add that many aliquots to the source labware layout.

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###########################
# Set up source materials #
###########################

# For each colourant
for colourant in Food_Colouring:
    # Get the total volume of colourant in the destination layout
    colourant_total_volume = Destination_Labware_Layout.get_total_volume_of_liquid(colourant)
    
    # Calculate the number of aliquots needed
    aliquots_needed = math.ceil(colourant_total_volume / Food_Colouring_Aliquot_Volume)
    
    # Add that many aliquots to the source layout
    for aliquot_n in range(0, aliquots_needed):
        # Get the next empty well
        well = Source_Labware_Layout.get_next_empty_well()
        
        # Add content to the empty well
        Source_Labware_Layout.add_content(
            Well = well,
            Reagent = colourant,
            Volume = 500 # uL
        )
###########################
# Set up source materials #
###########################

# For each colourant
for colourant in Food_Colouring:
    # Get the total volume of colourant in the destination layout
    colourant_total_volume = Destination_Labware_Layout.get_total_volume_of_liquid(colourant)
    
    # Calculate the number of aliquots needed
    aliquots_needed = math.ceil(colourant_total_volume / Food_Colouring_Aliquot_Volume)
    
    # Add that many aliquots to the source layout
    for aliquot_n in range(0, aliquots_needed):
        # Get the next empty well
        well = Source_Labware_Layout.get_next_empty_well()
        
        # Add content to the empty well
        Source_Labware_Layout.add_content(
            Well = well,
            Reagent = colourant,
            Volume = 500 # uL
        )

And once again the .print method can be used to check what has been added to the labware layout object

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Source_Labware_Layout.print()
Source_Labware_Layout.print()

Information for Source Labware
Plate Type: opentrons_24_aluminumblock_nest_1.5ml_snapcap
Well	Volume(uL)	Liquid Class	Reagent
A1	500.0		Unknown		Green
A2	500.0		Unknown		Green
A3	500.0		Unknown		Blue
A4	500.0		Unknown		Blue
A5	500.0		Unknown		Red
A6	500.0		Unknown		Red
B1	500.0		Unknown		Purple
B2	500.0		Unknown		Purple

Out[19]:

'A1\t500.0\t\tUnknown\t\tGreen\nA2\t500.0\t\tUnknown\t\tGreen\nA3\t500.0\t\tUnknown\t\tBlue\nA4\t500.0\t\tUnknown\t\tBlue\nA5\t500.0\t\tUnknown\t\tRed\nA6\t500.0\t\tUnknown\t\tRed\nB1\t500.0\t\tUnknown\t\tPurple\nB2\t500.0\t\tUnknown\t\tPurple\n'

Now let's add the food colouring to our source layout object. For this example, we'll simply pre-define where the source materials will be located.

Because we've defined which wells are available, we can use the get_next_empty_well method, which will simply return the next available well with no content.

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########################
# Add source materials #
########################

for colourant in Food_Colouring:
    well = Source_Labware_Layout.get_next_empty_well()

    Source_Labware_Layout.add_content(
        Well = well,
        Reagent = colourant,
        Volume = 500 # uL
    )
########################
# Add source materials #
########################

for colourant in Food_Colouring:
    well = Source_Labware_Layout.get_next_empty_well()

    Source_Labware_Layout.add_content(
        Well = well,
        Reagent = colourant,
        Volume = 500 # uL
    )

Load Labware¶

Now that we have the labware defined, we can load the labware to the Opentrons deck. This simply means letting the Opentrons know what labware is available, and which deck position it should be placed on. We can use OTP.next_empty_slot to get the next deck slot with nothing currently occupying that space.

Note that once again this piece of code uses the protocol object, and so won't work until we put it into the run function.

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################
# Load labware #
################

Source_Labware = OTP.load_labware_from_layout(
    Protocol = protocol,
    Labware_Layout = Source_Labware_Layout,
    deck_position = OTP.next_empty_slot(protocol),
)

Destination_Labware = OTP.load_labware_from_layout(
    Protocol = protocol,
    Labware_Layout = Destination_Labware_Layout,
    deck_position = OTP.next_empty_slot(protocol),
    custom_labware_dir = Custom_Labware_Directory
)
################
# Load labware #
################

Source_Labware = OTP.load_labware_from_layout(
    Protocol = protocol,
    Labware_Layout = Source_Labware_Layout,
    deck_position = OTP.next_empty_slot(protocol),
)

Destination_Labware = OTP.load_labware_from_layout(
    Protocol = protocol,
    Labware_Layout = Destination_Labware_Layout,
    deck_position = OTP.next_empty_slot(protocol),
    custom_labware_dir = Custom_Labware_Directory
)

Determine the Transfer Steps¶

We'll now use the OTP.dispense_from_aliquots function to help us determine the transfer steps to be performed by the Opentrons, and then use those to calculate the number of pipette tips required.

We're using the OTP.dispense_from_aliquots function here as we need to transfer liquid from a group of aliquots (the two aliquots of each food colourant) into a list of destination wells. If you need to define a set of transfer steps from one well to another in a specific way, then OTP.transfer_liquids might be more useful.

OTP.dispense_from_aliquots also has a lot more options for customising the liquid handling than we'll use here (such as aspiration speed, touch tip action, etc.). To see the full list of arguments the function takes, see the documentation.

As we just need to calculate the transfer actions at the moment, Calculate_Only is set to True. When we actually need to perform the liquid handling, we'll use Calculate_Only = False.

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#############################
# Caclulcate transfer steps #
#############################

# Dictionary to keep track of how many tips are needed

tips_required = {
    "p20": 0,
    "p300": 0,
    "p1000": 0,
}

# Dictionaries to store transfer lists, source locations, and destination locations
transfer_lists = {}
source_locations = {}
destination_locations = {}


# For each colourant
for colourant in Food_Colouring:

    # Get the locations of the colourant aliquots

    ## This gets the wells as strings (e.g. "A1", "A2")
    colourant_source_wells = Source_Labware_Layout.get_wells_containing_liquid(colourant)

    ## This converts the wells to Opentrons locations, which presents the Opentrons with XYZ co-ordinates
    colourant_source_locations = OTP.get_locations(
        Labware = Source_Labware,
        Wells = colourant_source_wells
    )

    # Get destination locations which contain the colourant

    ## This gets the wells as strings (e.g. "A1", "A2")
    destination_wells = Destination_Labware_Layout.get_wells_containing_liquid(colourant)

    ## This converts the wells to Opentrons locations, which presents the Opentrons with XYZ co-ordinates
    colourant_destination_locations = OTP.get_locations(
        Labware = Destination_Labware,
        Wells = destination_wells
    )

    # Get a list of transfer volumes
    transfer_volumes = [Destination_Labware_Layout.get_volume_of_liquid_in_well(colourant, well) for well in destination_wells]

    # Store this info in the dictionaries
    transfer_lists[colourant] = transfer_volumes.copy()
    source_locations[colourant] = colourant_source_locations.copy()
    destination_locations[colourant] = colourant_destination_locations.copy()


    # Calculate the transfer list for the colourant
    transfer_volumes, aliquot_source_order, colourant_destination_locations = OTP.dispense_from_aliquots(
        Protocol = protocol,
        Transfer_Volumes = transfer_volumes,
        Aliquot_Source_Locations = colourant_source_locations,
        Destinations = colourant_destination_locations,
        new_tip = True, # This will use a new tip for every transfer
        mix_after = (10, 20), # This will mix the destination well by pipetting up and down 10 times with a volumes of 20 uL
        Calculate_Only = True
    )

    # Calculate number of tips needed
    p20, p300, p1000 = OTP.calculate_tips_needed(
        protocol = protocol,
        transfers = transfer_volumes,
        new_tip = True,
    )

    tips_required["p20"] += p20
    tips_required["p300"] += p300
    tips_required["p1000"] += p1000
#############################
# Caclulcate transfer steps #
#############################

# Dictionary to keep track of how many tips are needed

tips_required = {
    "p20": 0,
    "p300": 0,
    "p1000": 0,
}

# Dictionaries to store transfer lists, source locations, and destination locations
transfer_lists = {}
source_locations = {}
destination_locations = {}


# For each colourant
for colourant in Food_Colouring:

    # Get the locations of the colourant aliquots

    ## This gets the wells as strings (e.g. "A1", "A2")
    colourant_source_wells = Source_Labware_Layout.get_wells_containing_liquid(colourant)

    ## This converts the wells to Opentrons locations, which presents the Opentrons with XYZ co-ordinates
    colourant_source_locations = OTP.get_locations(
        Labware = Source_Labware,
        Wells = colourant_source_wells
    )

    # Get destination locations which contain the colourant

    ## This gets the wells as strings (e.g. "A1", "A2")
    destination_wells = Destination_Labware_Layout.get_wells_containing_liquid(colourant)

    ## This converts the wells to Opentrons locations, which presents the Opentrons with XYZ co-ordinates
    colourant_destination_locations = OTP.get_locations(
        Labware = Destination_Labware,
        Wells = destination_wells
    )

    # Get a list of transfer volumes
    transfer_volumes = [Destination_Labware_Layout.get_volume_of_liquid_in_well(colourant, well) for well in destination_wells]

    # Store this info in the dictionaries
    transfer_lists[colourant] = transfer_volumes.copy()
    source_locations[colourant] = colourant_source_locations.copy()
    destination_locations[colourant] = colourant_destination_locations.copy()


    # Calculate the transfer list for the colourant
    transfer_volumes, aliquot_source_order, colourant_destination_locations = OTP.dispense_from_aliquots(
        Protocol = protocol,
        Transfer_Volumes = transfer_volumes,
        Aliquot_Source_Locations = colourant_source_locations,
        Destinations = colourant_destination_locations,
        new_tip = True, # This will use a new tip for every transfer
        mix_after = (10, 20), # This will mix the destination well by pipetting up and down 10 times with a volumes of 20 uL
        Calculate_Only = True
    )

    # Calculate number of tips needed
    p20, p300, p1000 = OTP.calculate_tips_needed(
        protocol = protocol,
        transfers = transfer_volumes,
        new_tip = True,
    )

    tips_required["p20"] += p20
    tips_required["p300"] += p300
    tips_required["p1000"] += p1000

Load Pipette Tips¶

Now, we'll finally load the pipette tips using the tips required variables we set up in the previous code block

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#####################
# Load pipette tips #
#####################

# Load tips for the p20
OTP.add_tip_boxes_to_pipettes(
    Protocol = protocol,
    Pipette_Type = "p20",
    Tip_Type = "opentrons_96_tiprack_20ul",
    Tips_Needed = tips_required["p20"]
)

# Load tips for the p300
OTP.add_tip_boxes_to_pipettes(
    Protocol = protocol,
    Pipette_Type = "p300",
    Tip_Type = "opentrons_96_tiprack_300ul",
    Tips_Needed = tips_required["p300"]
)
#####################
# Load pipette tips #
#####################

# Load tips for the p20
OTP.add_tip_boxes_to_pipettes(
    Protocol = protocol,
    Pipette_Type = "p20",
    Tip_Type = "opentrons_96_tiprack_20ul",
    Tips_Needed = tips_required["p20"]
)

# Load tips for the p300
OTP.add_tip_boxes_to_pipettes(
    Protocol = protocol,
    Pipette_Type = "p300",
    Tip_Type = "opentrons_96_tiprack_300ul",
    Tips_Needed = tips_required["p300"]
)

Liquid Handling¶

The last step is to actually perform the liquid handling. We'll use the OTP.dispense_from_aliquots function from earlier, but run it with Calculate_Only = False this time.

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###################
# Liquid Handling #
###################

for colourant in Food_Colouring:
    OTP.dispense_from_aliquots(
        Protocol = protocol,
        Transfer_Volumes = transfer_lists[colourant],
        Aliquot_Source_Locations = source_locations[colourant],
        Destinations = destination_locations[colourant],
        new_tip = True, # This will use a new tip for every transfer
        mix_after = (10, 20), # This will mix the destination well by pipetting up and down 10 times with a volumes of 20 uL
        Calculate_Only = False
    )
###################
# Liquid Handling #
###################

for colourant in Food_Colouring:
    OTP.dispense_from_aliquots(
        Protocol = protocol,
        Transfer_Volumes = transfer_lists[colourant],
        Aliquot_Source_Locations = source_locations[colourant],
        Destinations = destination_locations[colourant],
        new_tip = True, # This will use a new tip for every transfer
        mix_after = (10, 20), # This will mix the destination well by pipetting up and down 10 times with a volumes of 20 uL
        Calculate_Only = False
    )

Joining Everything Together¶

The code below is everything from above, put together into the run function:

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def run(protocol):
    #######################
    # General Information #
    #######################

    # Define the types of pipettes, and their location
    Pipettes = {
        "left": "p20_single_gen2",
        "right": "p300_single_gen2"
    }

    # For each pipette, define the pipette tips available to them
    Pipette_Tips = {
        "left": "opentrons_96_tiprack_20ul",
        "right": "opentrons_96_tiprack_300ul"
    }

    # List of source material

    Food_Colouring = [
        "Green",
        "Blue",
        "Red",
        "Purple"
    ]

    Food_Colouring_Aliquot_Volume = 500 # uL
    
    #################
    # Load Pipettes #
    #################

    for position in Pipettes.keys():
        protocol.load_instrument(
            Pipettes[position],
            position
        )
        
    ##################
    # Set up labware #
    ##################
    Custom_Labware_Directory = "../../../../data/custom_labware"

    Tube_Rack_Type = "opentrons_24_aluminumblock_nest_1.5ml_snapcap"
    Destination_Type = "greiner655087_96_wellplate_340ul"
    
    ##########################
    # Set up labware layouts #
    ##########################

    # Create the source object
    Source_Labware_Layout = BMS.Labware_Layout(
        Name = "Source Labware",
        Type = Tube_Rack_Type
    )

    # Define the labware's format (i.e. number of rows and columns)
    source_rows, source_columns = OTP.get_labware_format(
        labware_api_name = Source_Labware_Layout.type,
    )
    Source_Labware_Layout.define_format(source_rows, source_columns)
    
    # Create the destination object
    Destination_Labware_Layout = BMS.Labware_Layout(
        Name = "Destination Labware",
        Type = Destination_Type
    )

    # Define the labware's format (i.e. number of rows and columns)
    destination_rows, destination_columns = OTP.get_labware_format(
        labware_api_name = Destination_Labware_Layout.type,
        custom_labware_dir = Custom_Labware_Directory
    )
    Destination_Labware_Layout.define_format(destination_rows, destination_columns)
    
    Source_Labware_Layout.set_available_wells()
    Destination_Labware_Layout.set_available_wells()
    
    ################################
    # Define the destination state #
    ################################

    # Set up an empty list in which the colour mixtures required will be added
    Colour_Mixtures = []

    # Iterate through the different ratios
    # Iterate through the list of source colours provided to create the list of mixtures.
    for colour_1 in Food_Colouring:
        for colour_2 in Food_Colouring:
            # Ignore situations where colour_1 and colour_2 are the same
            if colour_1 == colour_2:
                continue
            else:
                # Add the two colours to the list of mixtures to prepare
                Colour_Mixtures.append([colour_1, colour_2])

    # Here, we'll print to OUT all of the mixtures which will be prepared
    for c in Colour_Mixtures:
        print(c)
        
    # Define the final destination state

    # For 3 replicates
    for rep in range(0, 3):
        # For each mixture
        for colour_1, colour_2 in Colour_Mixtures:

            # Get the next empty well
            well = Destination_Labware_Layout.get_next_empty_well()

            # Add the first colourant
            Destination_Labware_Layout.add_content(
                Well = well,
                Reagent = colour_1,
                Volume = 50 # uL
            )

            # And then the second
            Destination_Labware_Layout.add_content(
                Well = well,
                Reagent = colour_2,
                Volume = 50 # uL
            )

            # Can also label the well
            Destination_Labware_Layout.add_well_label(
                Well = well,
                Label = "Mixture: {}-{} Rep {}".format(colour_1, colour_2, rep)
            )
            
    Destination_Labware_Layout.print()
    
    ###########################
    # Set up source materials #
    ###########################

    # For each colourant
    for colourant in Food_Colouring:
        # Get the total volume of colourant in the destination layout
        colourant_total_volume = Destination_Labware_Layout.get_total_volume_of_liquid(colourant)

        # Calculate the number of aliquots needed
        aliquots_needed = math.ceil(colourant_total_volume / Food_Colouring_Aliquot_Volume)

        # Add that many aliquots to the source layout
        for aliquot_n in range(0, aliquots_needed):
            # Get the next empty well
            well = Source_Labware_Layout.get_next_empty_well()

            # Add content to the empty well
            Source_Labware_Layout.add_content(
                Well = well,
                Reagent = colourant,
                Volume = 500 # uL
            )
            
    Source_Labware_Layout.print()
    
    ########################
    # Add source materials #
    ########################

    for colourant in Food_Colouring:
        well = Source_Labware_Layout.get_next_empty_well()

        Source_Labware_Layout.add_content(
            Well = well,
            Reagent = colourant,
            Volume = 500 # uL
        )
        
    ################
    # Load labware #
    ################

    Source_Labware = OTP.load_labware_from_layout(
        Protocol = protocol,
        Labware_Layout = Source_Labware_Layout,
        deck_position = OTP.next_empty_slot(protocol),
    )

    Destination_Labware = OTP.load_labware_from_layout(
        Protocol = protocol,
        Labware_Layout = Destination_Labware_Layout,
        deck_position = OTP.next_empty_slot(protocol),
        custom_labware_dir = Custom_Labware_Directory
    )
    
    #############################
    # Caclulcate transfer steps #
    #############################

    # Dictionary to keep track of how many tips are needed

    tips_required = {
        "p20": 0,
        "p300": 0,
        "p1000": 0,
    }
    
    # Dictionaries to store transfer lists, source locations, and destination locations
    transfer_lists = {}
    source_locations = {}
    destination_locations = {}
    

    # For each colourant
    for colourant in Food_Colouring:

        # Get the locations of the colourant aliquots

        ## This gets the wells as strings (e.g. "A1", "A2")
        colourant_source_wells = Source_Labware_Layout.get_wells_containing_liquid(colourant)

        ## This converts the wells to Opentrons locations, which presents the Opentrons with XYZ co-ordinates
        colourant_source_locations = OTP.get_locations(
            Labware = Source_Labware,
            Wells = colourant_source_wells
        )

        # Get destination locations which contain the colourant

        ## This gets the wells as strings (e.g. "A1", "A2")
        destination_wells = Destination_Labware_Layout.get_wells_containing_liquid(colourant)

        ## This converts the wells to Opentrons locations, which presents the Opentrons with XYZ co-ordinates
        colourant_destination_locations = OTP.get_locations(
            Labware = Destination_Labware,
            Wells = destination_wells
        )

        # Get a list of transfer volumes
        transfer_volumes = [Destination_Labware_Layout.get_volume_of_liquid_in_well(colourant, well) for well in destination_wells]

        # Store this info in the dictionaries
        transfer_lists[colourant] = transfer_volumes.copy()
        source_locations[colourant] = colourant_source_locations.copy()
        destination_locations[colourant] = colourant_destination_locations.copy()
        
        
        # Calculate the transfer list for the colourant
        transfer_volumes, aliquot_source_order, colourant_destination_locations = OTP.dispense_from_aliquots(
            Protocol = protocol,
            Transfer_Volumes = transfer_volumes,
            Aliquot_Source_Locations = colourant_source_locations,
            Destinations = colourant_destination_locations,
            new_tip = True, # This will use a new tip for every transfer
            mix_after = (10, 20), # This will mix the destination well by pipetting up and down 10 times with a volumes of 20 uL
            Calculate_Only = True
        )

        # Calculate number of tips needed
        p20, p300, p1000 = OTP.calculate_tips_needed(
            protocol = protocol,
            transfers = transfer_volumes,
            new_tip = True,
        )

        tips_required["p20"] += p20
        tips_required["p300"] += p300
        tips_required["p1000"] += p1000
        
        

    #####################
    # Load pipette tips #
    #####################

    # Load tips for the p20
    OTP.add_tip_boxes_to_pipettes(
        Protocol = protocol,
        Pipette_Type = "p20",
        Tip_Type = "opentrons_96_tiprack_20ul",
        Tips_Needed = tips_required["p20"]
    )

    # Load tips for the p300
    OTP.add_tip_boxes_to_pipettes(
        Protocol = protocol,
        Pipette_Type = "p300",
        Tip_Type = "opentrons_96_tiprack_300ul",
        Tips_Needed = tips_required["p300"]
    )

    ###################
    # Liquid Handling #
    ###################

    for colourant in Food_Colouring:
        OTP.dispense_from_aliquots(
            Protocol = protocol,
            Transfer_Volumes = transfer_lists[colourant],
            Aliquot_Source_Locations = source_locations[colourant],
            Destinations = destination_locations[colourant],
            new_tip = True, # This will use a new tip for every transfer
            mix_after = (10, 20), # This will mix the destination well by pipetting up and down 10 times with a volumes of 20 uL
            Calculate_Only = False
        )
def run(protocol):
    #######################
    # General Information #
    #######################

    # Define the types of pipettes, and their location
    Pipettes = {
        "left": "p20_single_gen2",
        "right": "p300_single_gen2"
    }

    # For each pipette, define the pipette tips available to them
    Pipette_Tips = {
        "left": "opentrons_96_tiprack_20ul",
        "right": "opentrons_96_tiprack_300ul"
    }

    # List of source material

    Food_Colouring = [
        "Green",
        "Blue",
        "Red",
        "Purple"
    ]

    Food_Colouring_Aliquot_Volume = 500 # uL
    
    #################
    # Load Pipettes #
    #################

    for position in Pipettes.keys():
        protocol.load_instrument(
            Pipettes[position],
            position
        )
        
    ##################
    # Set up labware #
    ##################
    Custom_Labware_Directory = "../../../../data/custom_labware"

    Tube_Rack_Type = "opentrons_24_aluminumblock_nest_1.5ml_snapcap"
    Destination_Type = "greiner655087_96_wellplate_340ul"
    
    ##########################
    # Set up labware layouts #
    ##########################

    # Create the source object
    Source_Labware_Layout = BMS.Labware_Layout(
        Name = "Source Labware",
        Type = Tube_Rack_Type
    )

    # Define the labware's format (i.e. number of rows and columns)
    source_rows, source_columns = OTP.get_labware_format(
        labware_api_name = Source_Labware_Layout.type,
    )
    Source_Labware_Layout.define_format(source_rows, source_columns)
    
    # Create the destination object
    Destination_Labware_Layout = BMS.Labware_Layout(
        Name = "Destination Labware",
        Type = Destination_Type
    )

    # Define the labware's format (i.e. number of rows and columns)
    destination_rows, destination_columns = OTP.get_labware_format(
        labware_api_name = Destination_Labware_Layout.type,
        custom_labware_dir = Custom_Labware_Directory
    )
    Destination_Labware_Layout.define_format(destination_rows, destination_columns)
    
    Source_Labware_Layout.set_available_wells()
    Destination_Labware_Layout.set_available_wells()
    
    ################################
    # Define the destination state #
    ################################

    # Set up an empty list in which the colour mixtures required will be added
    Colour_Mixtures = []

    # Iterate through the different ratios
    # Iterate through the list of source colours provided to create the list of mixtures.
    for colour_1 in Food_Colouring:
        for colour_2 in Food_Colouring:
            # Ignore situations where colour_1 and colour_2 are the same
            if colour_1 == colour_2:
                continue
            else:
                # Add the two colours to the list of mixtures to prepare
                Colour_Mixtures.append([colour_1, colour_2])

    # Here, we'll print to OUT all of the mixtures which will be prepared
    for c in Colour_Mixtures:
        print(c)
        
    # Define the final destination state

    # For 3 replicates
    for rep in range(0, 3):
        # For each mixture
        for colour_1, colour_2 in Colour_Mixtures:

            # Get the next empty well
            well = Destination_Labware_Layout.get_next_empty_well()

            # Add the first colourant
            Destination_Labware_Layout.add_content(
                Well = well,
                Reagent = colour_1,
                Volume = 50 # uL
            )

            # And then the second
            Destination_Labware_Layout.add_content(
                Well = well,
                Reagent = colour_2,
                Volume = 50 # uL
            )

            # Can also label the well
            Destination_Labware_Layout.add_well_label(
                Well = well,
                Label = "Mixture: {}-{} Rep {}".format(colour_1, colour_2, rep)
            )
            
    Destination_Labware_Layout.print()
    
    ###########################
    # Set up source materials #
    ###########################

    # For each colourant
    for colourant in Food_Colouring:
        # Get the total volume of colourant in the destination layout
        colourant_total_volume = Destination_Labware_Layout.get_total_volume_of_liquid(colourant)

        # Calculate the number of aliquots needed
        aliquots_needed = math.ceil(colourant_total_volume / Food_Colouring_Aliquot_Volume)

        # Add that many aliquots to the source layout
        for aliquot_n in range(0, aliquots_needed):
            # Get the next empty well
            well = Source_Labware_Layout.get_next_empty_well()

            # Add content to the empty well
            Source_Labware_Layout.add_content(
                Well = well,
                Reagent = colourant,
                Volume = 500 # uL
            )
            
    Source_Labware_Layout.print()
    
    ########################
    # Add source materials #
    ########################

    for colourant in Food_Colouring:
        well = Source_Labware_Layout.get_next_empty_well()

        Source_Labware_Layout.add_content(
            Well = well,
            Reagent = colourant,
            Volume = 500 # uL
        )
        
    ################
    # Load labware #
    ################

    Source_Labware = OTP.load_labware_from_layout(
        Protocol = protocol,
        Labware_Layout = Source_Labware_Layout,
        deck_position = OTP.next_empty_slot(protocol),
    )

    Destination_Labware = OTP.load_labware_from_layout(
        Protocol = protocol,
        Labware_Layout = Destination_Labware_Layout,
        deck_position = OTP.next_empty_slot(protocol),
        custom_labware_dir = Custom_Labware_Directory
    )
    
    #############################
    # Caclulcate transfer steps #
    #############################

    # Dictionary to keep track of how many tips are needed

    tips_required = {
        "p20": 0,
        "p300": 0,
        "p1000": 0,
    }
    
    # Dictionaries to store transfer lists, source locations, and destination locations
    transfer_lists = {}
    source_locations = {}
    destination_locations = {}
    

    # For each colourant
    for colourant in Food_Colouring:

        # Get the locations of the colourant aliquots

        ## This gets the wells as strings (e.g. "A1", "A2")
        colourant_source_wells = Source_Labware_Layout.get_wells_containing_liquid(colourant)

        ## This converts the wells to Opentrons locations, which presents the Opentrons with XYZ co-ordinates
        colourant_source_locations = OTP.get_locations(
            Labware = Source_Labware,
            Wells = colourant_source_wells
        )

        # Get destination locations which contain the colourant

        ## This gets the wells as strings (e.g. "A1", "A2")
        destination_wells = Destination_Labware_Layout.get_wells_containing_liquid(colourant)

        ## This converts the wells to Opentrons locations, which presents the Opentrons with XYZ co-ordinates
        colourant_destination_locations = OTP.get_locations(
            Labware = Destination_Labware,
            Wells = destination_wells
        )

        # Get a list of transfer volumes
        transfer_volumes = [Destination_Labware_Layout.get_volume_of_liquid_in_well(colourant, well) for well in destination_wells]

        # Store this info in the dictionaries
        transfer_lists[colourant] = transfer_volumes.copy()
        source_locations[colourant] = colourant_source_locations.copy()
        destination_locations[colourant] = colourant_destination_locations.copy()
        
        
        # Calculate the transfer list for the colourant
        transfer_volumes, aliquot_source_order, colourant_destination_locations = OTP.dispense_from_aliquots(
            Protocol = protocol,
            Transfer_Volumes = transfer_volumes,
            Aliquot_Source_Locations = colourant_source_locations,
            Destinations = colourant_destination_locations,
            new_tip = True, # This will use a new tip for every transfer
            mix_after = (10, 20), # This will mix the destination well by pipetting up and down 10 times with a volumes of 20 uL
            Calculate_Only = True
        )

        # Calculate number of tips needed
        p20, p300, p1000 = OTP.calculate_tips_needed(
            protocol = protocol,
            transfers = transfer_volumes,
            new_tip = True,
        )

        tips_required["p20"] += p20
        tips_required["p300"] += p300
        tips_required["p1000"] += p1000
        
        

    #####################
    # Load pipette tips #
    #####################

    # Load tips for the p20
    OTP.add_tip_boxes_to_pipettes(
        Protocol = protocol,
        Pipette_Type = "p20",
        Tip_Type = "opentrons_96_tiprack_20ul",
        Tips_Needed = tips_required["p20"]
    )

    # Load tips for the p300
    OTP.add_tip_boxes_to_pipettes(
        Protocol = protocol,
        Pipette_Type = "p300",
        Tip_Type = "opentrons_96_tiprack_300ul",
        Tips_Needed = tips_required["p300"]
    )

    ###################
    # Liquid Handling #
    ###################

    for colourant in Food_Colouring:
        OTP.dispense_from_aliquots(
            Protocol = protocol,
            Transfer_Volumes = transfer_lists[colourant],
            Aliquot_Source_Locations = source_locations[colourant],
            Destinations = destination_locations[colourant],
            new_tip = True, # This will use a new tip for every transfer
            mix_after = (10, 20), # This will mix the destination well by pipetting up and down 10 times with a volumes of 20 uL
            Calculate_Only = False
        )

Simulation¶

The code below can be used to simulate the protocol. Note that this MUST be removed before trying to run the script on the Opentrons.

In [26]:

            
                Copied!
                
                    
                    
                
                

        
#####################################################################
# Use this cell if simulating the protocol, otherwise comment it out #
#####################################################################

#########################################################################################################
# IMPORTANT - the protocol will not upload to the opentrons if this cell is not commented out or removed #
#########################################################################################################

from opentrons import simulate as OT2 # This line simulates the protocol
# Get the correct api version
protocol = OT2.get_protocol_api(metadata["apiLevel"])
# Home the pipetting head
protocol.home()
# Call the 'run' function to run the protocol
p = run(protocol)
for line in protocol.commands():
    print(line)
#####################################################################
# Use this cell if simulating the protocol, otherwise comment it out #
#####################################################################

#########################################################################################################
# IMPORTANT - the protocol will not upload to the opentrons if this cell is not commented out or removed #
#########################################################################################################

from opentrons import simulate as OT2 # This line simulates the protocol
# Get the correct api version
protocol = OT2.get_protocol_api(metadata["apiLevel"])
# Home the pipetting head
protocol.home()
# Call the 'run' function to run the protocol
p = run(protocol)
for line in protocol.commands():
    print(line)

C:\Users\bradl\.opentrons\robot_settings.json not found. Loading defaults
C:\Users\bradl\.opentrons\deck_calibration.json not found. Loading defaults

['Green', 'Blue']
['Green', 'Red']
['Green', 'Purple']
['Blue', 'Green']
['Blue', 'Red']
['Blue', 'Purple']
['Red', 'Green']
['Red', 'Blue']
['Red', 'Purple']
['Purple', 'Green']
['Purple', 'Blue']
['Purple', 'Red']
Information for Destination Labware
Plate Type: greiner655087_96_wellplate_340ul
Well	Volume(uL)	Liquid Class	Reagent
A1	50.0		Unknown		Green
A1	50.0		Unknown		Blue
A2	50.0		Unknown		Green
A2	50.0		Unknown		Red
A3	50.0		Unknown		Green
A3	50.0		Unknown		Purple
A4	50.0		Unknown		Blue
A4	50.0		Unknown		Green
A5	50.0		Unknown		Blue
A5	50.0		Unknown		Red
A6	50.0		Unknown		Blue
A6	50.0		Unknown		Purple
A7	50.0		Unknown		Red
A7	50.0		Unknown		Green
A8	50.0		Unknown		Red
A8	50.0		Unknown		Blue
A9	50.0		Unknown		Red
A9	50.0		Unknown		Purple
A10	50.0		Unknown		Purple
A10	50.0		Unknown		Green
A11	50.0		Unknown		Purple
A11	50.0		Unknown		Blue
A12	50.0		Unknown		Purple
A12	50.0		Unknown		Red
B1	50.0		Unknown		Green
B1	50.0		Unknown		Blue
B2	50.0		Unknown		Green
B2	50.0		Unknown		Red
B3	50.0		Unknown		Green
B3	50.0		Unknown		Purple
B4	50.0		Unknown		Blue
B4	50.0		Unknown		Green
B5	50.0		Unknown		Blue
B5	50.0		Unknown		Red
B6	50.0		Unknown		Blue
B6	50.0		Unknown		Purple
B7	50.0		Unknown		Red
B7	50.0		Unknown		Green
B8	50.0		Unknown		Red
B8	50.0		Unknown		Blue
B9	50.0		Unknown		Red
B9	50.0		Unknown		Purple
B10	50.0		Unknown		Purple
B10	50.0		Unknown		Green
B11	50.0		Unknown		Purple
B11	50.0		Unknown		Blue
B12	50.0		Unknown		Purple
B12	50.0		Unknown		Red
C1	50.0		Unknown		Green
C1	50.0		Unknown		Blue
C2	50.0		Unknown		Green
C2	50.0		Unknown		Red
C3	50.0		Unknown		Green
C3	50.0		Unknown		Purple
C4	50.0		Unknown		Blue
C4	50.0		Unknown		Green
C5	50.0		Unknown		Blue
C5	50.0		Unknown		Red
C6	50.0		Unknown		Blue
C6	50.0		Unknown		Purple
C7	50.0		Unknown		Red
C7	50.0		Unknown		Green
C8	50.0		Unknown		Red
C8	50.0		Unknown		Blue
C9	50.0		Unknown		Red
C9	50.0		Unknown		Purple
C10	50.0		Unknown		Purple
C10	50.0		Unknown		Green
C11	50.0		Unknown		Purple
C11	50.0		Unknown		Blue
C12	50.0		Unknown		Purple
C12	50.0		Unknown		Red
Information for Source Labware
Plate Type: opentrons_24_aluminumblock_nest_1.5ml_snapcap
Well	Volume(uL)	Liquid Class	Reagent
A1	500.0		Unknown		Green
A2	500.0		Unknown		Green
A3	500.0		Unknown		Blue
A4	500.0		Unknown		Blue
A5	500.0		Unknown		Red
A6	500.0		Unknown		Red
B1	500.0		Unknown		Purple
B2	500.0		Unknown		Purple
Picking up tip from A1 of Opentrons 96 Tip Rack 300 µL on 3
Moving to A1 of Source Labware on 1
Aspirating 50.0 uL from A1 of Source Labware on 1 at 92.86 uL/sec
Moving to A1 of Destination Labware on 2
Dispensing 50.0 uL into A1 of Destination Labware on 2 at 92.86 uL/sec
Mixing 10 times with a volume of 20.0 ul
Aspirating 20.0 uL from A1 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A1 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A1 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A1 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A1 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A1 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A1 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A1 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A1 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A1 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A1 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A1 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A1 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A1 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A1 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A1 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A1 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A1 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A1 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A1 of Destination Labware on 2 at 92.86 uL/sec
Dropping tip into A1 of Opentrons Fixed Trash on 12
Picking up tip from B1 of Opentrons 96 Tip Rack 300 µL on 3
Moving to A2 of Source Labware on 1
Aspirating 50.0 uL from A2 of Source Labware on 1 at 92.86 uL/sec
Moving to A2 of Destination Labware on 2
Dispensing 50.0 uL into A2 of Destination Labware on 2 at 92.86 uL/sec
Mixing 10 times with a volume of 20.0 ul
Aspirating 20.0 uL from A2 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A2 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A2 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A2 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A2 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A2 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A2 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A2 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A2 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A2 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A2 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A2 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A2 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A2 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A2 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A2 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A2 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A2 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A2 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A2 of Destination Labware on 2 at 92.86 uL/sec
Dropping tip into A1 of Opentrons Fixed Trash on 12
Picking up tip from C1 of Opentrons 96 Tip Rack 300 µL on 3
Moving to B3 of Source Labware on 1
Aspirating 50.0 uL from B3 of Source Labware on 1 at 92.86 uL/sec
Moving to A3 of Destination Labware on 2
Dispensing 50.0 uL into A3 of Destination Labware on 2 at 92.86 uL/sec
Mixing 10 times with a volume of 20.0 ul
Aspirating 20.0 uL from A3 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A3 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A3 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A3 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A3 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A3 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A3 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A3 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A3 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A3 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A3 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A3 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A3 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A3 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A3 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A3 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A3 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A3 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A3 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A3 of Destination Labware on 2 at 92.86 uL/sec
Dropping tip into A1 of Opentrons Fixed Trash on 12
Picking up tip from D1 of Opentrons 96 Tip Rack 300 µL on 3
Moving to A1 of Source Labware on 1
Aspirating 50.0 uL from A1 of Source Labware on 1 at 92.86 uL/sec
Moving to A4 of Destination Labware on 2
Dispensing 50.0 uL into A4 of Destination Labware on 2 at 92.86 uL/sec
Mixing 10 times with a volume of 20.0 ul
Aspirating 20.0 uL from A4 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A4 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A4 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A4 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A4 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A4 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A4 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A4 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A4 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A4 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A4 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A4 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A4 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A4 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A4 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A4 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A4 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A4 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A4 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A4 of Destination Labware on 2 at 92.86 uL/sec
Dropping tip into A1 of Opentrons Fixed Trash on 12
Picking up tip from E1 of Opentrons 96 Tip Rack 300 µL on 3
Moving to A2 of Source Labware on 1
Aspirating 50.0 uL from A2 of Source Labware on 1 at 92.86 uL/sec
Moving to A7 of Destination Labware on 2
Dispensing 50.0 uL into A7 of Destination Labware on 2 at 92.86 uL/sec
Mixing 10 times with a volume of 20.0 ul
Aspirating 20.0 uL from A7 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A7 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A7 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A7 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A7 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A7 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A7 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A7 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A7 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A7 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A7 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A7 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A7 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A7 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A7 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A7 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A7 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A7 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A7 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A7 of Destination Labware on 2 at 92.86 uL/sec
Dropping tip into A1 of Opentrons Fixed Trash on 12
Picking up tip from F1 of Opentrons 96 Tip Rack 300 µL on 3
Moving to B3 of Source Labware on 1
Aspirating 50.0 uL from B3 of Source Labware on 1 at 92.86 uL/sec
Moving to A10 of Destination Labware on 2
Dispensing 50.0 uL into A10 of Destination Labware on 2 at 92.86 uL/sec
Mixing 10 times with a volume of 20.0 ul
Aspirating 20.0 uL from A10 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A10 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A10 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A10 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A10 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A10 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A10 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A10 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A10 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A10 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A10 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A10 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A10 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A10 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A10 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A10 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A10 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A10 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A10 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A10 of Destination Labware on 2 at 92.86 uL/sec
Dropping tip into A1 of Opentrons Fixed Trash on 12
Picking up tip from G1 of Opentrons 96 Tip Rack 300 µL on 3
Moving to A1 of Source Labware on 1
Aspirating 50.0 uL from A1 of Source Labware on 1 at 92.86 uL/sec
Moving to B1 of Destination Labware on 2
Dispensing 50.0 uL into B1 of Destination Labware on 2 at 92.86 uL/sec
Mixing 10 times with a volume of 20.0 ul
Aspirating 20.0 uL from B1 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B1 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B1 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B1 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B1 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B1 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B1 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B1 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B1 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B1 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B1 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B1 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B1 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B1 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B1 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B1 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B1 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B1 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B1 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B1 of Destination Labware on 2 at 92.86 uL/sec
Dropping tip into A1 of Opentrons Fixed Trash on 12
Picking up tip from H1 of Opentrons 96 Tip Rack 300 µL on 3
Moving to A2 of Source Labware on 1
Aspirating 50.0 uL from A2 of Source Labware on 1 at 92.86 uL/sec
Moving to B2 of Destination Labware on 2
Dispensing 50.0 uL into B2 of Destination Labware on 2 at 92.86 uL/sec
Mixing 10 times with a volume of 20.0 ul
Aspirating 20.0 uL from B2 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B2 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B2 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B2 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B2 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B2 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B2 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B2 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B2 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B2 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B2 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B2 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B2 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B2 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B2 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B2 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B2 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B2 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B2 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B2 of Destination Labware on 2 at 92.86 uL/sec
Dropping tip into A1 of Opentrons Fixed Trash on 12
Picking up tip from A2 of Opentrons 96 Tip Rack 300 µL on 3
Moving to B3 of Source Labware on 1
Aspirating 50.0 uL from B3 of Source Labware on 1 at 92.86 uL/sec
Moving to B3 of Destination Labware on 2
Dispensing 50.0 uL into B3 of Destination Labware on 2 at 92.86 uL/sec
Mixing 10 times with a volume of 20.0 ul
Aspirating 20.0 uL from B3 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B3 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B3 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B3 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B3 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B3 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B3 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B3 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B3 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B3 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B3 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B3 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B3 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B3 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B3 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B3 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B3 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B3 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B3 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B3 of Destination Labware on 2 at 92.86 uL/sec
Dropping tip into A1 of Opentrons Fixed Trash on 12
Picking up tip from B2 of Opentrons 96 Tip Rack 300 µL on 3
Moving to A1 of Source Labware on 1
Aspirating 50.0 uL from A1 of Source Labware on 1 at 92.86 uL/sec
Moving to B4 of Destination Labware on 2
Dispensing 50.0 uL into B4 of Destination Labware on 2 at 92.86 uL/sec
Mixing 10 times with a volume of 20.0 ul
Aspirating 20.0 uL from B4 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B4 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B4 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B4 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B4 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B4 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B4 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B4 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B4 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B4 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B4 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B4 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B4 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B4 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B4 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B4 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B4 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B4 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B4 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B4 of Destination Labware on 2 at 92.86 uL/sec
Dropping tip into A1 of Opentrons Fixed Trash on 12
Picking up tip from C2 of Opentrons 96 Tip Rack 300 µL on 3
Moving to A2 of Source Labware on 1
Aspirating 50.0 uL from A2 of Source Labware on 1 at 92.86 uL/sec
Moving to B7 of Destination Labware on 2
Dispensing 50.0 uL into B7 of Destination Labware on 2 at 92.86 uL/sec
Mixing 10 times with a volume of 20.0 ul
Aspirating 20.0 uL from B7 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B7 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B7 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B7 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B7 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B7 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B7 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B7 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B7 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B7 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B7 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B7 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B7 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B7 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B7 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B7 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B7 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B7 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B7 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B7 of Destination Labware on 2 at 92.86 uL/sec
Dropping tip into A1 of Opentrons Fixed Trash on 12
Picking up tip from D2 of Opentrons 96 Tip Rack 300 µL on 3
Moving to B3 of Source Labware on 1
Aspirating 50.0 uL from B3 of Source Labware on 1 at 92.86 uL/sec
Moving to B10 of Destination Labware on 2
Dispensing 50.0 uL into B10 of Destination Labware on 2 at 92.86 uL/sec
Mixing 10 times with a volume of 20.0 ul
Aspirating 20.0 uL from B10 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B10 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B10 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B10 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B10 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B10 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B10 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B10 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B10 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B10 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B10 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B10 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B10 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B10 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B10 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B10 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B10 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B10 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B10 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B10 of Destination Labware on 2 at 92.86 uL/sec
Dropping tip into A1 of Opentrons Fixed Trash on 12
Picking up tip from E2 of Opentrons 96 Tip Rack 300 µL on 3
Moving to A1 of Source Labware on 1
Aspirating 50.0 uL from A1 of Source Labware on 1 at 92.86 uL/sec
Moving to C1 of Destination Labware on 2
Dispensing 50.0 uL into C1 of Destination Labware on 2 at 92.86 uL/sec
Mixing 10 times with a volume of 20.0 ul
Aspirating 20.0 uL from C1 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C1 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C1 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C1 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C1 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C1 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C1 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C1 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C1 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C1 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C1 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C1 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C1 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C1 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C1 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C1 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C1 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C1 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C1 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C1 of Destination Labware on 2 at 92.86 uL/sec
Dropping tip into A1 of Opentrons Fixed Trash on 12
Picking up tip from F2 of Opentrons 96 Tip Rack 300 µL on 3
Moving to A2 of Source Labware on 1
Aspirating 50.0 uL from A2 of Source Labware on 1 at 92.86 uL/sec
Moving to C2 of Destination Labware on 2
Dispensing 50.0 uL into C2 of Destination Labware on 2 at 92.86 uL/sec
Mixing 10 times with a volume of 20.0 ul
Aspirating 20.0 uL from C2 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C2 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C2 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C2 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C2 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C2 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C2 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C2 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C2 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C2 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C2 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C2 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C2 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C2 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C2 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C2 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C2 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C2 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C2 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C2 of Destination Labware on 2 at 92.86 uL/sec
Dropping tip into A1 of Opentrons Fixed Trash on 12
Picking up tip from G2 of Opentrons 96 Tip Rack 300 µL on 3
Moving to B3 of Source Labware on 1
Aspirating 50.0 uL from B3 of Source Labware on 1 at 92.86 uL/sec
Moving to C3 of Destination Labware on 2
Dispensing 50.0 uL into C3 of Destination Labware on 2 at 92.86 uL/sec
Mixing 10 times with a volume of 20.0 ul
Aspirating 20.0 uL from C3 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C3 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C3 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C3 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C3 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C3 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C3 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C3 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C3 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C3 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C3 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C3 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C3 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C3 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C3 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C3 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C3 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C3 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C3 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C3 of Destination Labware on 2 at 92.86 uL/sec
Dropping tip into A1 of Opentrons Fixed Trash on 12
Picking up tip from H2 of Opentrons 96 Tip Rack 300 µL on 3
Moving to A1 of Source Labware on 1
Aspirating 50.0 uL from A1 of Source Labware on 1 at 92.86 uL/sec
Moving to C4 of Destination Labware on 2
Dispensing 50.0 uL into C4 of Destination Labware on 2 at 92.86 uL/sec
Mixing 10 times with a volume of 20.0 ul
Aspirating 20.0 uL from C4 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C4 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C4 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C4 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C4 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C4 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C4 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C4 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C4 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C4 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C4 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C4 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C4 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C4 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C4 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C4 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C4 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C4 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C4 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C4 of Destination Labware on 2 at 92.86 uL/sec
Dropping tip into A1 of Opentrons Fixed Trash on 12
Picking up tip from A3 of Opentrons 96 Tip Rack 300 µL on 3
Moving to A2 of Source Labware on 1
Aspirating 50.0 uL from A2 of Source Labware on 1 at 92.86 uL/sec
Moving to C7 of Destination Labware on 2
Dispensing 50.0 uL into C7 of Destination Labware on 2 at 92.86 uL/sec
Mixing 10 times with a volume of 20.0 ul
Aspirating 20.0 uL from C7 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C7 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C7 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C7 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C7 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C7 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C7 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C7 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C7 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C7 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C7 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C7 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C7 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C7 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C7 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C7 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C7 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C7 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C7 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C7 of Destination Labware on 2 at 92.86 uL/sec
Dropping tip into A1 of Opentrons Fixed Trash on 12
Picking up tip from B3 of Opentrons 96 Tip Rack 300 µL on 3
Moving to B3 of Source Labware on 1
Aspirating 50.0 uL from B3 of Source Labware on 1 at 92.86 uL/sec
Moving to C10 of Destination Labware on 2
Dispensing 50.0 uL into C10 of Destination Labware on 2 at 92.86 uL/sec
Mixing 10 times with a volume of 20.0 ul
Aspirating 20.0 uL from C10 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C10 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C10 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C10 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C10 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C10 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C10 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C10 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C10 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C10 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C10 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C10 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C10 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C10 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C10 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C10 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C10 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C10 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C10 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C10 of Destination Labware on 2 at 92.86 uL/sec
Dropping tip into A1 of Opentrons Fixed Trash on 12
Picking up tip from C3 of Opentrons 96 Tip Rack 300 µL on 3
Moving to A3 of Source Labware on 1
Aspirating 50.0 uL from A3 of Source Labware on 1 at 92.86 uL/sec
Moving to A1 of Destination Labware on 2
Dispensing 50.0 uL into A1 of Destination Labware on 2 at 92.86 uL/sec
Mixing 10 times with a volume of 20.0 ul
Aspirating 20.0 uL from A1 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A1 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A1 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A1 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A1 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A1 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A1 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A1 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A1 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A1 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A1 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A1 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A1 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A1 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A1 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A1 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A1 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A1 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A1 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A1 of Destination Labware on 2 at 92.86 uL/sec
Dropping tip into A1 of Opentrons Fixed Trash on 12
Picking up tip from D3 of Opentrons 96 Tip Rack 300 µL on 3
Moving to A4 of Source Labware on 1
Aspirating 50.0 uL from A4 of Source Labware on 1 at 92.86 uL/sec
Moving to A4 of Destination Labware on 2
Dispensing 50.0 uL into A4 of Destination Labware on 2 at 92.86 uL/sec
Mixing 10 times with a volume of 20.0 ul
Aspirating 20.0 uL from A4 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A4 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A4 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A4 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A4 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A4 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A4 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A4 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A4 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A4 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A4 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A4 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A4 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A4 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A4 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A4 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A4 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A4 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A4 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A4 of Destination Labware on 2 at 92.86 uL/sec
Dropping tip into A1 of Opentrons Fixed Trash on 12
Picking up tip from E3 of Opentrons 96 Tip Rack 300 µL on 3
Moving to B4 of Source Labware on 1
Aspirating 50.0 uL from B4 of Source Labware on 1 at 92.86 uL/sec
Moving to A5 of Destination Labware on 2
Dispensing 50.0 uL into A5 of Destination Labware on 2 at 92.86 uL/sec
Mixing 10 times with a volume of 20.0 ul
Aspirating 20.0 uL from A5 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A5 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A5 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A5 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A5 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A5 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A5 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A5 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A5 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A5 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A5 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A5 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A5 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A5 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A5 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A5 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A5 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A5 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A5 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A5 of Destination Labware on 2 at 92.86 uL/sec
Dropping tip into A1 of Opentrons Fixed Trash on 12
Picking up tip from F3 of Opentrons 96 Tip Rack 300 µL on 3
Moving to A3 of Source Labware on 1
Aspirating 50.0 uL from A3 of Source Labware on 1 at 92.86 uL/sec
Moving to A6 of Destination Labware on 2
Dispensing 50.0 uL into A6 of Destination Labware on 2 at 92.86 uL/sec
Mixing 10 times with a volume of 20.0 ul
Aspirating 20.0 uL from A6 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A6 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A6 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A6 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A6 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A6 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A6 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A6 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A6 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A6 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A6 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A6 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A6 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A6 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A6 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A6 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A6 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A6 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A6 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A6 of Destination Labware on 2 at 92.86 uL/sec
Dropping tip into A1 of Opentrons Fixed Trash on 12
Picking up tip from G3 of Opentrons 96 Tip Rack 300 µL on 3
Moving to A4 of Source Labware on 1
Aspirating 50.0 uL from A4 of Source Labware on 1 at 92.86 uL/sec
Moving to A8 of Destination Labware on 2
Dispensing 50.0 uL into A8 of Destination Labware on 2 at 92.86 uL/sec
Mixing 10 times with a volume of 20.0 ul
Aspirating 20.0 uL from A8 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A8 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A8 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A8 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A8 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A8 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A8 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A8 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A8 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A8 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A8 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A8 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A8 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A8 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A8 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A8 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A8 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A8 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A8 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A8 of Destination Labware on 2 at 92.86 uL/sec
Dropping tip into A1 of Opentrons Fixed Trash on 12
Picking up tip from H3 of Opentrons 96 Tip Rack 300 µL on 3
Moving to B4 of Source Labware on 1
Aspirating 50.0 uL from B4 of Source Labware on 1 at 92.86 uL/sec
Moving to A11 of Destination Labware on 2
Dispensing 50.0 uL into A11 of Destination Labware on 2 at 92.86 uL/sec
Mixing 10 times with a volume of 20.0 ul
Aspirating 20.0 uL from A11 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A11 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A11 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A11 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A11 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A11 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A11 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A11 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A11 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A11 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A11 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A11 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A11 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A11 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A11 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A11 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A11 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A11 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A11 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A11 of Destination Labware on 2 at 92.86 uL/sec
Dropping tip into A1 of Opentrons Fixed Trash on 12
Picking up tip from A4 of Opentrons 96 Tip Rack 300 µL on 3
Moving to A3 of Source Labware on 1
Aspirating 50.0 uL from A3 of Source Labware on 1 at 92.86 uL/sec
Moving to B1 of Destination Labware on 2
Dispensing 50.0 uL into B1 of Destination Labware on 2 at 92.86 uL/sec
Mixing 10 times with a volume of 20.0 ul
Aspirating 20.0 uL from B1 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B1 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B1 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B1 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B1 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B1 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B1 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B1 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B1 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B1 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B1 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B1 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B1 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B1 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B1 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B1 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B1 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B1 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B1 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B1 of Destination Labware on 2 at 92.86 uL/sec
Dropping tip into A1 of Opentrons Fixed Trash on 12
Picking up tip from B4 of Opentrons 96 Tip Rack 300 µL on 3
Moving to A4 of Source Labware on 1
Aspirating 50.0 uL from A4 of Source Labware on 1 at 92.86 uL/sec
Moving to B4 of Destination Labware on 2
Dispensing 50.0 uL into B4 of Destination Labware on 2 at 92.86 uL/sec
Mixing 10 times with a volume of 20.0 ul
Aspirating 20.0 uL from B4 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B4 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B4 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B4 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B4 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B4 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B4 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B4 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B4 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B4 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B4 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B4 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B4 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B4 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B4 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B4 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B4 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B4 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B4 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B4 of Destination Labware on 2 at 92.86 uL/sec
Dropping tip into A1 of Opentrons Fixed Trash on 12
Picking up tip from C4 of Opentrons 96 Tip Rack 300 µL on 3
Moving to B4 of Source Labware on 1
Aspirating 50.0 uL from B4 of Source Labware on 1 at 92.86 uL/sec
Moving to B5 of Destination Labware on 2
Dispensing 50.0 uL into B5 of Destination Labware on 2 at 92.86 uL/sec
Mixing 10 times with a volume of 20.0 ul
Aspirating 20.0 uL from B5 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B5 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B5 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B5 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B5 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B5 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B5 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B5 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B5 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B5 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B5 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B5 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B5 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B5 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B5 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B5 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B5 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B5 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B5 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B5 of Destination Labware on 2 at 92.86 uL/sec
Dropping tip into A1 of Opentrons Fixed Trash on 12
Picking up tip from D4 of Opentrons 96 Tip Rack 300 µL on 3
Moving to A3 of Source Labware on 1
Aspirating 50.0 uL from A3 of Source Labware on 1 at 92.86 uL/sec
Moving to B6 of Destination Labware on 2
Dispensing 50.0 uL into B6 of Destination Labware on 2 at 92.86 uL/sec
Mixing 10 times with a volume of 20.0 ul
Aspirating 20.0 uL from B6 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B6 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B6 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B6 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B6 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B6 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B6 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B6 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B6 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B6 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B6 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B6 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B6 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B6 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B6 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B6 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B6 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B6 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B6 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B6 of Destination Labware on 2 at 92.86 uL/sec
Dropping tip into A1 of Opentrons Fixed Trash on 12
Picking up tip from E4 of Opentrons 96 Tip Rack 300 µL on 3
Moving to A4 of Source Labware on 1
Aspirating 50.0 uL from A4 of Source Labware on 1 at 92.86 uL/sec
Moving to B8 of Destination Labware on 2
Dispensing 50.0 uL into B8 of Destination Labware on 2 at 92.86 uL/sec
Mixing 10 times with a volume of 20.0 ul
Aspirating 20.0 uL from B8 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B8 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B8 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B8 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B8 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B8 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B8 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B8 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B8 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B8 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B8 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B8 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B8 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B8 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B8 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B8 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B8 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B8 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B8 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B8 of Destination Labware on 2 at 92.86 uL/sec
Dropping tip into A1 of Opentrons Fixed Trash on 12
Picking up tip from F4 of Opentrons 96 Tip Rack 300 µL on 3
Moving to B4 of Source Labware on 1
Aspirating 50.0 uL from B4 of Source Labware on 1 at 92.86 uL/sec
Moving to B11 of Destination Labware on 2
Dispensing 50.0 uL into B11 of Destination Labware on 2 at 92.86 uL/sec
Mixing 10 times with a volume of 20.0 ul
Aspirating 20.0 uL from B11 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B11 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B11 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B11 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B11 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B11 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B11 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B11 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B11 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B11 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B11 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B11 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B11 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B11 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B11 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B11 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B11 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B11 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B11 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B11 of Destination Labware on 2 at 92.86 uL/sec
Dropping tip into A1 of Opentrons Fixed Trash on 12
Picking up tip from G4 of Opentrons 96 Tip Rack 300 µL on 3
Moving to A3 of Source Labware on 1
Aspirating 50.0 uL from A3 of Source Labware on 1 at 92.86 uL/sec
Moving to C1 of Destination Labware on 2
Dispensing 50.0 uL into C1 of Destination Labware on 2 at 92.86 uL/sec
Mixing 10 times with a volume of 20.0 ul
Aspirating 20.0 uL from C1 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C1 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C1 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C1 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C1 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C1 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C1 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C1 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C1 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C1 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C1 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C1 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C1 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C1 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C1 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C1 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C1 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C1 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C1 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C1 of Destination Labware on 2 at 92.86 uL/sec
Dropping tip into A1 of Opentrons Fixed Trash on 12
Picking up tip from H4 of Opentrons 96 Tip Rack 300 µL on 3
Moving to A4 of Source Labware on 1
Aspirating 50.0 uL from A4 of Source Labware on 1 at 92.86 uL/sec
Moving to C4 of Destination Labware on 2
Dispensing 50.0 uL into C4 of Destination Labware on 2 at 92.86 uL/sec
Mixing 10 times with a volume of 20.0 ul
Aspirating 20.0 uL from C4 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C4 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C4 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C4 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C4 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C4 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C4 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C4 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C4 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C4 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C4 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C4 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C4 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C4 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C4 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C4 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C4 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C4 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C4 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C4 of Destination Labware on 2 at 92.86 uL/sec
Dropping tip into A1 of Opentrons Fixed Trash on 12
Picking up tip from A5 of Opentrons 96 Tip Rack 300 µL on 3
Moving to B4 of Source Labware on 1
Aspirating 50.0 uL from B4 of Source Labware on 1 at 92.86 uL/sec
Moving to C5 of Destination Labware on 2
Dispensing 50.0 uL into C5 of Destination Labware on 2 at 92.86 uL/sec
Mixing 10 times with a volume of 20.0 ul
Aspirating 20.0 uL from C5 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C5 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C5 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C5 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C5 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C5 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C5 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C5 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C5 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C5 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C5 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C5 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C5 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C5 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C5 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C5 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C5 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C5 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C5 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C5 of Destination Labware on 2 at 92.86 uL/sec
Dropping tip into A1 of Opentrons Fixed Trash on 12
Picking up tip from B5 of Opentrons 96 Tip Rack 300 µL on 3
Moving to A3 of Source Labware on 1
Aspirating 50.0 uL from A3 of Source Labware on 1 at 92.86 uL/sec
Moving to C6 of Destination Labware on 2
Dispensing 50.0 uL into C6 of Destination Labware on 2 at 92.86 uL/sec
Mixing 10 times with a volume of 20.0 ul
Aspirating 20.0 uL from C6 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C6 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C6 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C6 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C6 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C6 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C6 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C6 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C6 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C6 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C6 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C6 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C6 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C6 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C6 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C6 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C6 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C6 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C6 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C6 of Destination Labware on 2 at 92.86 uL/sec
Dropping tip into A1 of Opentrons Fixed Trash on 12
Picking up tip from C5 of Opentrons 96 Tip Rack 300 µL on 3
Moving to A4 of Source Labware on 1
Aspirating 50.0 uL from A4 of Source Labware on 1 at 92.86 uL/sec
Moving to C8 of Destination Labware on 2
Dispensing 50.0 uL into C8 of Destination Labware on 2 at 92.86 uL/sec
Mixing 10 times with a volume of 20.0 ul
Aspirating 20.0 uL from C8 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C8 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C8 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C8 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C8 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C8 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C8 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C8 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C8 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C8 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C8 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C8 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C8 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C8 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C8 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C8 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C8 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C8 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C8 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C8 of Destination Labware on 2 at 92.86 uL/sec
Dropping tip into A1 of Opentrons Fixed Trash on 12
Picking up tip from D5 of Opentrons 96 Tip Rack 300 µL on 3
Moving to B4 of Source Labware on 1
Aspirating 50.0 uL from B4 of Source Labware on 1 at 92.86 uL/sec
Moving to C11 of Destination Labware on 2
Dispensing 50.0 uL into C11 of Destination Labware on 2 at 92.86 uL/sec
Mixing 10 times with a volume of 20.0 ul
Aspirating 20.0 uL from C11 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C11 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C11 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C11 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C11 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C11 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C11 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C11 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C11 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C11 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C11 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C11 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C11 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C11 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C11 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C11 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C11 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C11 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C11 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C11 of Destination Labware on 2 at 92.86 uL/sec
Dropping tip into A1 of Opentrons Fixed Trash on 12
Picking up tip from E5 of Opentrons 96 Tip Rack 300 µL on 3
Moving to A5 of Source Labware on 1
Aspirating 50.0 uL from A5 of Source Labware on 1 at 92.86 uL/sec
Moving to A2 of Destination Labware on 2
Dispensing 50.0 uL into A2 of Destination Labware on 2 at 92.86 uL/sec
Mixing 10 times with a volume of 20.0 ul
Aspirating 20.0 uL from A2 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A2 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A2 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A2 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A2 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A2 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A2 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A2 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A2 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A2 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A2 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A2 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A2 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A2 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A2 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A2 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A2 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A2 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A2 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A2 of Destination Labware on 2 at 92.86 uL/sec
Dropping tip into A1 of Opentrons Fixed Trash on 12
Picking up tip from F5 of Opentrons 96 Tip Rack 300 µL on 3
Moving to A6 of Source Labware on 1
Aspirating 50.0 uL from A6 of Source Labware on 1 at 92.86 uL/sec
Moving to A5 of Destination Labware on 2
Dispensing 50.0 uL into A5 of Destination Labware on 2 at 92.86 uL/sec
Mixing 10 times with a volume of 20.0 ul
Aspirating 20.0 uL from A5 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A5 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A5 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A5 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A5 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A5 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A5 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A5 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A5 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A5 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A5 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A5 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A5 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A5 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A5 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A5 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A5 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A5 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A5 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A5 of Destination Labware on 2 at 92.86 uL/sec
Dropping tip into A1 of Opentrons Fixed Trash on 12
Picking up tip from G5 of Opentrons 96 Tip Rack 300 µL on 3
Moving to B5 of Source Labware on 1
Aspirating 50.0 uL from B5 of Source Labware on 1 at 92.86 uL/sec
Moving to A7 of Destination Labware on 2
Dispensing 50.0 uL into A7 of Destination Labware on 2 at 92.86 uL/sec
Mixing 10 times with a volume of 20.0 ul
Aspirating 20.0 uL from A7 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A7 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A7 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A7 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A7 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A7 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A7 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A7 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A7 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A7 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A7 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A7 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A7 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A7 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A7 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A7 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A7 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A7 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A7 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A7 of Destination Labware on 2 at 92.86 uL/sec
Dropping tip into A1 of Opentrons Fixed Trash on 12
Picking up tip from H5 of Opentrons 96 Tip Rack 300 µL on 3
Moving to A5 of Source Labware on 1
Aspirating 50.0 uL from A5 of Source Labware on 1 at 92.86 uL/sec
Moving to A8 of Destination Labware on 2
Dispensing 50.0 uL into A8 of Destination Labware on 2 at 92.86 uL/sec
Mixing 10 times with a volume of 20.0 ul
Aspirating 20.0 uL from A8 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A8 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A8 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A8 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A8 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A8 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A8 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A8 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A8 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A8 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A8 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A8 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A8 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A8 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A8 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A8 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A8 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A8 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A8 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A8 of Destination Labware on 2 at 92.86 uL/sec
Dropping tip into A1 of Opentrons Fixed Trash on 12
Picking up tip from A6 of Opentrons 96 Tip Rack 300 µL on 3
Moving to A6 of Source Labware on 1
Aspirating 50.0 uL from A6 of Source Labware on 1 at 92.86 uL/sec
Moving to A9 of Destination Labware on 2
Dispensing 50.0 uL into A9 of Destination Labware on 2 at 92.86 uL/sec
Mixing 10 times with a volume of 20.0 ul
Aspirating 20.0 uL from A9 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A9 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A9 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A9 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A9 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A9 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A9 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A9 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A9 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A9 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A9 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A9 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A9 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A9 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A9 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A9 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A9 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A9 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A9 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A9 of Destination Labware on 2 at 92.86 uL/sec
Dropping tip into A1 of Opentrons Fixed Trash on 12
Picking up tip from B6 of Opentrons 96 Tip Rack 300 µL on 3
Moving to B5 of Source Labware on 1
Aspirating 50.0 uL from B5 of Source Labware on 1 at 92.86 uL/sec
Moving to A12 of Destination Labware on 2
Dispensing 50.0 uL into A12 of Destination Labware on 2 at 92.86 uL/sec
Mixing 10 times with a volume of 20.0 ul
Aspirating 20.0 uL from A12 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A12 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A12 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A12 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A12 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A12 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A12 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A12 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A12 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A12 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A12 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A12 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A12 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A12 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A12 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A12 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A12 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A12 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A12 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A12 of Destination Labware on 2 at 92.86 uL/sec
Dropping tip into A1 of Opentrons Fixed Trash on 12
Picking up tip from C6 of Opentrons 96 Tip Rack 300 µL on 3
Moving to A5 of Source Labware on 1
Aspirating 50.0 uL from A5 of Source Labware on 1 at 92.86 uL/sec
Moving to B2 of Destination Labware on 2
Dispensing 50.0 uL into B2 of Destination Labware on 2 at 92.86 uL/sec
Mixing 10 times with a volume of 20.0 ul
Aspirating 20.0 uL from B2 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B2 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B2 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B2 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B2 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B2 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B2 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B2 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B2 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B2 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B2 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B2 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B2 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B2 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B2 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B2 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B2 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B2 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B2 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B2 of Destination Labware on 2 at 92.86 uL/sec
Dropping tip into A1 of Opentrons Fixed Trash on 12
Picking up tip from D6 of Opentrons 96 Tip Rack 300 µL on 3
Moving to A6 of Source Labware on 1
Aspirating 50.0 uL from A6 of Source Labware on 1 at 92.86 uL/sec
Moving to B5 of Destination Labware on 2
Dispensing 50.0 uL into B5 of Destination Labware on 2 at 92.86 uL/sec
Mixing 10 times with a volume of 20.0 ul
Aspirating 20.0 uL from B5 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B5 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B5 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B5 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B5 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B5 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B5 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B5 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B5 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B5 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B5 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B5 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B5 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B5 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B5 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B5 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B5 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B5 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B5 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B5 of Destination Labware on 2 at 92.86 uL/sec
Dropping tip into A1 of Opentrons Fixed Trash on 12
Picking up tip from E6 of Opentrons 96 Tip Rack 300 µL on 3
Moving to B5 of Source Labware on 1
Aspirating 50.0 uL from B5 of Source Labware on 1 at 92.86 uL/sec
Moving to B7 of Destination Labware on 2
Dispensing 50.0 uL into B7 of Destination Labware on 2 at 92.86 uL/sec
Mixing 10 times with a volume of 20.0 ul
Aspirating 20.0 uL from B7 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B7 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B7 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B7 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B7 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B7 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B7 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B7 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B7 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B7 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B7 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B7 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B7 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B7 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B7 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B7 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B7 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B7 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B7 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B7 of Destination Labware on 2 at 92.86 uL/sec
Dropping tip into A1 of Opentrons Fixed Trash on 12
Picking up tip from F6 of Opentrons 96 Tip Rack 300 µL on 3
Moving to A5 of Source Labware on 1
Aspirating 50.0 uL from A5 of Source Labware on 1 at 92.86 uL/sec
Moving to B8 of Destination Labware on 2
Dispensing 50.0 uL into B8 of Destination Labware on 2 at 92.86 uL/sec
Mixing 10 times with a volume of 20.0 ul
Aspirating 20.0 uL from B8 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B8 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B8 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B8 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B8 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B8 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B8 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B8 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B8 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B8 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B8 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B8 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B8 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B8 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B8 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B8 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B8 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B8 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B8 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B8 of Destination Labware on 2 at 92.86 uL/sec
Dropping tip into A1 of Opentrons Fixed Trash on 12
Picking up tip from G6 of Opentrons 96 Tip Rack 300 µL on 3
Moving to A6 of Source Labware on 1
Aspirating 50.0 uL from A6 of Source Labware on 1 at 92.86 uL/sec
Moving to B9 of Destination Labware on 2
Dispensing 50.0 uL into B9 of Destination Labware on 2 at 92.86 uL/sec
Mixing 10 times with a volume of 20.0 ul
Aspirating 20.0 uL from B9 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B9 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B9 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B9 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B9 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B9 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B9 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B9 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B9 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B9 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B9 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B9 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B9 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B9 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B9 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B9 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B9 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B9 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B9 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B9 of Destination Labware on 2 at 92.86 uL/sec
Dropping tip into A1 of Opentrons Fixed Trash on 12
Picking up tip from H6 of Opentrons 96 Tip Rack 300 µL on 3
Moving to B5 of Source Labware on 1
Aspirating 50.0 uL from B5 of Source Labware on 1 at 92.86 uL/sec
Moving to B12 of Destination Labware on 2
Dispensing 50.0 uL into B12 of Destination Labware on 2 at 92.86 uL/sec
Mixing 10 times with a volume of 20.0 ul
Aspirating 20.0 uL from B12 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B12 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B12 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B12 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B12 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B12 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B12 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B12 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B12 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B12 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B12 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B12 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B12 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B12 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B12 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B12 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B12 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B12 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B12 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B12 of Destination Labware on 2 at 92.86 uL/sec
Dropping tip into A1 of Opentrons Fixed Trash on 12
Picking up tip from A7 of Opentrons 96 Tip Rack 300 µL on 3
Moving to A5 of Source Labware on 1
Aspirating 50.0 uL from A5 of Source Labware on 1 at 92.86 uL/sec
Moving to C2 of Destination Labware on 2
Dispensing 50.0 uL into C2 of Destination Labware on 2 at 92.86 uL/sec
Mixing 10 times with a volume of 20.0 ul
Aspirating 20.0 uL from C2 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C2 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C2 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C2 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C2 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C2 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C2 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C2 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C2 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C2 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C2 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C2 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C2 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C2 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C2 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C2 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C2 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C2 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C2 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C2 of Destination Labware on 2 at 92.86 uL/sec
Dropping tip into A1 of Opentrons Fixed Trash on 12
Picking up tip from B7 of Opentrons 96 Tip Rack 300 µL on 3
Moving to A6 of Source Labware on 1
Aspirating 50.0 uL from A6 of Source Labware on 1 at 92.86 uL/sec
Moving to C5 of Destination Labware on 2
Dispensing 50.0 uL into C5 of Destination Labware on 2 at 92.86 uL/sec
Mixing 10 times with a volume of 20.0 ul
Aspirating 20.0 uL from C5 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C5 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C5 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C5 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C5 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C5 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C5 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C5 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C5 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C5 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C5 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C5 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C5 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C5 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C5 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C5 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C5 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C5 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C5 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C5 of Destination Labware on 2 at 92.86 uL/sec
Dropping tip into A1 of Opentrons Fixed Trash on 12
Picking up tip from C7 of Opentrons 96 Tip Rack 300 µL on 3
Moving to B5 of Source Labware on 1
Aspirating 50.0 uL from B5 of Source Labware on 1 at 92.86 uL/sec
Moving to C7 of Destination Labware on 2
Dispensing 50.0 uL into C7 of Destination Labware on 2 at 92.86 uL/sec
Mixing 10 times with a volume of 20.0 ul
Aspirating 20.0 uL from C7 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C7 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C7 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C7 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C7 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C7 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C7 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C7 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C7 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C7 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C7 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C7 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C7 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C7 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C7 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C7 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C7 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C7 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C7 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C7 of Destination Labware on 2 at 92.86 uL/sec
Dropping tip into A1 of Opentrons Fixed Trash on 12
Picking up tip from D7 of Opentrons 96 Tip Rack 300 µL on 3
Moving to A5 of Source Labware on 1
Aspirating 50.0 uL from A5 of Source Labware on 1 at 92.86 uL/sec
Moving to C8 of Destination Labware on 2
Dispensing 50.0 uL into C8 of Destination Labware on 2 at 92.86 uL/sec
Mixing 10 times with a volume of 20.0 ul
Aspirating 20.0 uL from C8 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C8 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C8 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C8 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C8 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C8 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C8 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C8 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C8 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C8 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C8 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C8 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C8 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C8 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C8 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C8 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C8 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C8 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C8 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C8 of Destination Labware on 2 at 92.86 uL/sec
Dropping tip into A1 of Opentrons Fixed Trash on 12
Picking up tip from E7 of Opentrons 96 Tip Rack 300 µL on 3
Moving to A6 of Source Labware on 1
Aspirating 50.0 uL from A6 of Source Labware on 1 at 92.86 uL/sec
Moving to C9 of Destination Labware on 2
Dispensing 50.0 uL into C9 of Destination Labware on 2 at 92.86 uL/sec
Mixing 10 times with a volume of 20.0 ul
Aspirating 20.0 uL from C9 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C9 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C9 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C9 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C9 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C9 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C9 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C9 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C9 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C9 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C9 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C9 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C9 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C9 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C9 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C9 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C9 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C9 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C9 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C9 of Destination Labware on 2 at 92.86 uL/sec
Dropping tip into A1 of Opentrons Fixed Trash on 12
Picking up tip from F7 of Opentrons 96 Tip Rack 300 µL on 3
Moving to B5 of Source Labware on 1
Aspirating 50.0 uL from B5 of Source Labware on 1 at 92.86 uL/sec
Moving to C12 of Destination Labware on 2
Dispensing 50.0 uL into C12 of Destination Labware on 2 at 92.86 uL/sec
Mixing 10 times with a volume of 20.0 ul
Aspirating 20.0 uL from C12 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C12 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C12 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C12 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C12 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C12 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C12 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C12 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C12 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C12 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C12 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C12 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C12 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C12 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C12 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C12 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C12 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C12 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C12 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C12 of Destination Labware on 2 at 92.86 uL/sec
Dropping tip into A1 of Opentrons Fixed Trash on 12
Picking up tip from G7 of Opentrons 96 Tip Rack 300 µL on 3
Moving to B1 of Source Labware on 1
Aspirating 50.0 uL from B1 of Source Labware on 1 at 92.86 uL/sec
Moving to A3 of Destination Labware on 2
Dispensing 50.0 uL into A3 of Destination Labware on 2 at 92.86 uL/sec
Mixing 10 times with a volume of 20.0 ul
Aspirating 20.0 uL from A3 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A3 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A3 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A3 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A3 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A3 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A3 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A3 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A3 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A3 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A3 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A3 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A3 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A3 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A3 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A3 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A3 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A3 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A3 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A3 of Destination Labware on 2 at 92.86 uL/sec
Dropping tip into A1 of Opentrons Fixed Trash on 12
Picking up tip from H7 of Opentrons 96 Tip Rack 300 µL on 3
Moving to B2 of Source Labware on 1
Aspirating 50.0 uL from B2 of Source Labware on 1 at 92.86 uL/sec
Moving to A6 of Destination Labware on 2
Dispensing 50.0 uL into A6 of Destination Labware on 2 at 92.86 uL/sec
Mixing 10 times with a volume of 20.0 ul
Aspirating 20.0 uL from A6 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A6 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A6 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A6 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A6 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A6 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A6 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A6 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A6 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A6 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A6 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A6 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A6 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A6 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A6 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A6 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A6 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A6 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A6 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A6 of Destination Labware on 2 at 92.86 uL/sec
Dropping tip into A1 of Opentrons Fixed Trash on 12
Picking up tip from A8 of Opentrons 96 Tip Rack 300 µL on 3
Moving to B6 of Source Labware on 1
Aspirating 50.0 uL from B6 of Source Labware on 1 at 92.86 uL/sec
Moving to A9 of Destination Labware on 2
Dispensing 50.0 uL into A9 of Destination Labware on 2 at 92.86 uL/sec
Mixing 10 times with a volume of 20.0 ul
Aspirating 20.0 uL from A9 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A9 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A9 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A9 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A9 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A9 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A9 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A9 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A9 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A9 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A9 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A9 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A9 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A9 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A9 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A9 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A9 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A9 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A9 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A9 of Destination Labware on 2 at 92.86 uL/sec
Dropping tip into A1 of Opentrons Fixed Trash on 12
Picking up tip from B8 of Opentrons 96 Tip Rack 300 µL on 3
Moving to B1 of Source Labware on 1
Aspirating 50.0 uL from B1 of Source Labware on 1 at 92.86 uL/sec
Moving to A10 of Destination Labware on 2
Dispensing 50.0 uL into A10 of Destination Labware on 2 at 92.86 uL/sec
Mixing 10 times with a volume of 20.0 ul
Aspirating 20.0 uL from A10 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A10 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A10 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A10 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A10 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A10 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A10 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A10 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A10 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A10 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A10 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A10 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A10 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A10 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A10 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A10 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A10 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A10 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A10 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A10 of Destination Labware on 2 at 92.86 uL/sec
Dropping tip into A1 of Opentrons Fixed Trash on 12
Picking up tip from C8 of Opentrons 96 Tip Rack 300 µL on 3
Moving to B2 of Source Labware on 1
Aspirating 50.0 uL from B2 of Source Labware on 1 at 92.86 uL/sec
Moving to A11 of Destination Labware on 2
Dispensing 50.0 uL into A11 of Destination Labware on 2 at 92.86 uL/sec
Mixing 10 times with a volume of 20.0 ul
Aspirating 20.0 uL from A11 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A11 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A11 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A11 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A11 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A11 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A11 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A11 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A11 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A11 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A11 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A11 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A11 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A11 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A11 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A11 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A11 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A11 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A11 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A11 of Destination Labware on 2 at 92.86 uL/sec
Dropping tip into A1 of Opentrons Fixed Trash on 12
Picking up tip from D8 of Opentrons 96 Tip Rack 300 µL on 3
Moving to B6 of Source Labware on 1
Aspirating 50.0 uL from B6 of Source Labware on 1 at 92.86 uL/sec
Moving to A12 of Destination Labware on 2
Dispensing 50.0 uL into A12 of Destination Labware on 2 at 92.86 uL/sec
Mixing 10 times with a volume of 20.0 ul
Aspirating 20.0 uL from A12 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A12 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A12 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A12 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A12 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A12 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A12 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A12 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A12 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A12 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A12 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A12 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A12 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A12 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A12 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A12 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A12 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A12 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from A12 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into A12 of Destination Labware on 2 at 92.86 uL/sec
Dropping tip into A1 of Opentrons Fixed Trash on 12
Picking up tip from E8 of Opentrons 96 Tip Rack 300 µL on 3
Moving to B1 of Source Labware on 1
Aspirating 50.0 uL from B1 of Source Labware on 1 at 92.86 uL/sec
Moving to B3 of Destination Labware on 2
Dispensing 50.0 uL into B3 of Destination Labware on 2 at 92.86 uL/sec
Mixing 10 times with a volume of 20.0 ul
Aspirating 20.0 uL from B3 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B3 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B3 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B3 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B3 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B3 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B3 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B3 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B3 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B3 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B3 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B3 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B3 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B3 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B3 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B3 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B3 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B3 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B3 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B3 of Destination Labware on 2 at 92.86 uL/sec
Dropping tip into A1 of Opentrons Fixed Trash on 12
Picking up tip from F8 of Opentrons 96 Tip Rack 300 µL on 3
Moving to B2 of Source Labware on 1
Aspirating 50.0 uL from B2 of Source Labware on 1 at 92.86 uL/sec
Moving to B6 of Destination Labware on 2
Dispensing 50.0 uL into B6 of Destination Labware on 2 at 92.86 uL/sec
Mixing 10 times with a volume of 20.0 ul
Aspirating 20.0 uL from B6 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B6 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B6 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B6 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B6 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B6 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B6 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B6 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B6 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B6 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B6 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B6 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B6 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B6 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B6 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B6 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B6 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B6 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B6 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B6 of Destination Labware on 2 at 92.86 uL/sec
Dropping tip into A1 of Opentrons Fixed Trash on 12
Picking up tip from G8 of Opentrons 96 Tip Rack 300 µL on 3
Moving to B6 of Source Labware on 1
Aspirating 50.0 uL from B6 of Source Labware on 1 at 92.86 uL/sec
Moving to B9 of Destination Labware on 2
Dispensing 50.0 uL into B9 of Destination Labware on 2 at 92.86 uL/sec
Mixing 10 times with a volume of 20.0 ul
Aspirating 20.0 uL from B9 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B9 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B9 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B9 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B9 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B9 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B9 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B9 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B9 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B9 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B9 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B9 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B9 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B9 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B9 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B9 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B9 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B9 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B9 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B9 of Destination Labware on 2 at 92.86 uL/sec
Dropping tip into A1 of Opentrons Fixed Trash on 12
Picking up tip from H8 of Opentrons 96 Tip Rack 300 µL on 3
Moving to B1 of Source Labware on 1
Aspirating 50.0 uL from B1 of Source Labware on 1 at 92.86 uL/sec
Moving to B10 of Destination Labware on 2
Dispensing 50.0 uL into B10 of Destination Labware on 2 at 92.86 uL/sec
Mixing 10 times with a volume of 20.0 ul
Aspirating 20.0 uL from B10 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B10 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B10 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B10 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B10 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B10 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B10 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B10 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B10 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B10 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B10 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B10 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B10 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B10 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B10 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B10 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B10 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B10 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B10 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B10 of Destination Labware on 2 at 92.86 uL/sec
Dropping tip into A1 of Opentrons Fixed Trash on 12
Picking up tip from A9 of Opentrons 96 Tip Rack 300 µL on 3
Moving to B2 of Source Labware on 1
Aspirating 50.0 uL from B2 of Source Labware on 1 at 92.86 uL/sec
Moving to B11 of Destination Labware on 2
Dispensing 50.0 uL into B11 of Destination Labware on 2 at 92.86 uL/sec
Mixing 10 times with a volume of 20.0 ul
Aspirating 20.0 uL from B11 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B11 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B11 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B11 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B11 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B11 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B11 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B11 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B11 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B11 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B11 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B11 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B11 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B11 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B11 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B11 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B11 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B11 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B11 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B11 of Destination Labware on 2 at 92.86 uL/sec
Dropping tip into A1 of Opentrons Fixed Trash on 12
Picking up tip from B9 of Opentrons 96 Tip Rack 300 µL on 3
Moving to B6 of Source Labware on 1
Aspirating 50.0 uL from B6 of Source Labware on 1 at 92.86 uL/sec
Moving to B12 of Destination Labware on 2
Dispensing 50.0 uL into B12 of Destination Labware on 2 at 92.86 uL/sec
Mixing 10 times with a volume of 20.0 ul
Aspirating 20.0 uL from B12 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B12 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B12 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B12 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B12 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B12 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B12 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B12 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B12 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B12 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B12 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B12 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B12 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B12 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B12 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B12 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B12 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B12 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from B12 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into B12 of Destination Labware on 2 at 92.86 uL/sec
Dropping tip into A1 of Opentrons Fixed Trash on 12
Picking up tip from C9 of Opentrons 96 Tip Rack 300 µL on 3
Moving to B1 of Source Labware on 1
Aspirating 50.0 uL from B1 of Source Labware on 1 at 92.86 uL/sec
Moving to C3 of Destination Labware on 2
Dispensing 50.0 uL into C3 of Destination Labware on 2 at 92.86 uL/sec
Mixing 10 times with a volume of 20.0 ul
Aspirating 20.0 uL from C3 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C3 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C3 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C3 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C3 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C3 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C3 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C3 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C3 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C3 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C3 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C3 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C3 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C3 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C3 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C3 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C3 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C3 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C3 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C3 of Destination Labware on 2 at 92.86 uL/sec
Dropping tip into A1 of Opentrons Fixed Trash on 12
Picking up tip from D9 of Opentrons 96 Tip Rack 300 µL on 3
Moving to B2 of Source Labware on 1
Aspirating 50.0 uL from B2 of Source Labware on 1 at 92.86 uL/sec
Moving to C6 of Destination Labware on 2
Dispensing 50.0 uL into C6 of Destination Labware on 2 at 92.86 uL/sec
Mixing 10 times with a volume of 20.0 ul
Aspirating 20.0 uL from C6 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C6 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C6 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C6 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C6 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C6 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C6 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C6 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C6 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C6 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C6 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C6 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C6 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C6 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C6 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C6 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C6 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C6 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C6 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C6 of Destination Labware on 2 at 92.86 uL/sec
Dropping tip into A1 of Opentrons Fixed Trash on 12
Picking up tip from E9 of Opentrons 96 Tip Rack 300 µL on 3
Moving to B6 of Source Labware on 1
Aspirating 50.0 uL from B6 of Source Labware on 1 at 92.86 uL/sec
Moving to C9 of Destination Labware on 2
Dispensing 50.0 uL into C9 of Destination Labware on 2 at 92.86 uL/sec
Mixing 10 times with a volume of 20.0 ul
Aspirating 20.0 uL from C9 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C9 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C9 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C9 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C9 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C9 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C9 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C9 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C9 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C9 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C9 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C9 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C9 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C9 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C9 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C9 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C9 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C9 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C9 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C9 of Destination Labware on 2 at 92.86 uL/sec
Dropping tip into A1 of Opentrons Fixed Trash on 12
Picking up tip from F9 of Opentrons 96 Tip Rack 300 µL on 3
Moving to B1 of Source Labware on 1
Aspirating 50.0 uL from B1 of Source Labware on 1 at 92.86 uL/sec
Moving to C10 of Destination Labware on 2
Dispensing 50.0 uL into C10 of Destination Labware on 2 at 92.86 uL/sec
Mixing 10 times with a volume of 20.0 ul
Aspirating 20.0 uL from C10 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C10 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C10 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C10 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C10 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C10 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C10 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C10 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C10 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C10 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C10 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C10 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C10 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C10 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C10 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C10 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C10 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C10 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C10 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C10 of Destination Labware on 2 at 92.86 uL/sec
Dropping tip into A1 of Opentrons Fixed Trash on 12
Picking up tip from G9 of Opentrons 96 Tip Rack 300 µL on 3
Moving to B2 of Source Labware on 1
Aspirating 50.0 uL from B2 of Source Labware on 1 at 92.86 uL/sec
Moving to C11 of Destination Labware on 2
Dispensing 50.0 uL into C11 of Destination Labware on 2 at 92.86 uL/sec
Mixing 10 times with a volume of 20.0 ul
Aspirating 20.0 uL from C11 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C11 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C11 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C11 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C11 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C11 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C11 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C11 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C11 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C11 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C11 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C11 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C11 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C11 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C11 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C11 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C11 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C11 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C11 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C11 of Destination Labware on 2 at 92.86 uL/sec
Dropping tip into A1 of Opentrons Fixed Trash on 12
Picking up tip from H9 of Opentrons 96 Tip Rack 300 µL on 3
Moving to B6 of Source Labware on 1
Aspirating 50.0 uL from B6 of Source Labware on 1 at 92.86 uL/sec
Moving to C12 of Destination Labware on 2
Dispensing 50.0 uL into C12 of Destination Labware on 2 at 92.86 uL/sec
Mixing 10 times with a volume of 20.0 ul
Aspirating 20.0 uL from C12 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C12 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C12 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C12 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C12 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C12 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C12 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C12 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C12 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C12 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C12 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C12 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C12 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C12 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C12 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C12 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C12 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C12 of Destination Labware on 2 at 92.86 uL/sec
Aspirating 20.0 uL from C12 of Destination Labware on 2 at 92.86 uL/sec
Dispensing 20.0 uL into C12 of Destination Labware on 2 at 92.86 uL/sec
Dropping tip into A1 of Opentrons Fixed Trash on 12

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